Differential expression of TcR-CD3 zeta as evidence for altered immunoregulation in preeclamptic versus normotensive women - 28/08/11
, Paul W Whitecar, MD b, Susan Orton, PhD c, Kim A Boggess, MD a, Douglas D Taylor, PhD d
Reprint requests to: Garrett Lam, MD, 214 MacNider Building, CB7516, Chapel Hill, NC 27703.Chapel Hill and Fort Bragg, NC, and Louisville, Ky
Abstract |
Objective |
The study was undertaken to exhibit and quantify the difference in modulation of CD3-zeta protein (an integral component of the T-cell receptor) in preeclamptic and normotensive women.
Study design |
Serum was collected from 10 preeclamptic and 10 normotensive women at ≥37 weeks' gestation on admission. Jurkat E-61 cells were incubated with the sera (20% volume to volume) and analyzed with Western immunoblot using mouse monoclonal CD3-zeta antibody. Enhanced chemiluminescence and densitometry were used to qualitatively measure zeta expression of the cells. A de novo flow cytometry assay was developed to quantify the difference in CD3-zeta expression of these cells. Comparsions were performed by t test (P<.05 was significant).
Results |
Preeclamptic patient sera produced a 2.4-fold increase in CD3-zeta expression than normotensive patients on Western blot (P<.01). Flow cytometry showed that preeclamptic sera had a 1.4-fold higher expression of CD3-zeta compared with normotensive patients (P<.0003).
Conclusion |
TcR/CD3-zeta expression is normally suppressed in pregnancy. Loss of this suppression occurs in preeclamptic patients, implying increased T-cell function.
Le texte complet de cet article est disponible en PDF.Keywords : TcR-CD3 zeta protein, T-cell function, preeclampsia
Plan
 | Supported in part by a grant from the Preeclampsia Foundation. Presented at the Twenty-Third Annual Meeting of the Society for Maternal-Fetal Medicine, San Francisco, Calif, February 3-8, 2003. The opinions and assertions contained herein are the private views of the authors and are not to be construed as official or reflecting the views of the Department of the Army or the Department of Defense. |
Vol 189 - N° 3
P. 843-847 - septembre 2003 Retour au numéro
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