Bile acids increase response and expression of human myometrial oxytocin receptor - 28/08/11
Abstract |
OBJECTIVE: We tested the hypothesis that during intrahepatic cholestasis of pregnancy bile acids activate the myometrial oxytocin receptor pathway.
STUDY DESIGN: Myometrial sensitivity to oxytocin and oxytocin-receptor messenger RNA and protein level was investigated. The ability of cholic acid to mediate such changes was evaluated.
RESULTS: Cholestasis patients required lesser oxytocin to elicit four uterine contractions in 10 minutes (1.3±0.6 vs 3.6±0.8 U, P<.05, n=7) and had lower in vitro ED50 (1.6×10−10 mol/L vs 1.0×10−8 mol/L, P<.05, n=7) than controls. The 24-hour incubation of control myometrial strips (n=7) with cholic acid (20 μmol/L) increased oxytocin sensitivity. Incubation of cultured myometrial cells (n=5) with cholic acid increased oxytocin-receptor expression (messenger RNA and protein).
CONCLUSION: We demonstrate that during intrahepatic cholestasis of pregnancy, an activation of the oxytocin receptor pathway occurs. This event seems to be the result of a cholic acid–mediated increase in oxytocin-receptor expression.
Le texte complet de cet article est disponible en PDF.Keywords : Intrahepatic cholestasis of pregnancy, oxytocin receptor
Plan
 | Supported by FONDECYT 1980964, DIPUC 97II/12 CE, DIPUC 3811102, and a Research and Development Grant from Gynopharm Laboratories I/98. Reprints not available from the authors. |
Vol 189 - N° 2
P. 577-582 - août 2003 Retour au numéro
Article précédent
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