Diagnosis of natural rubber latex allergy by the Basotest method - 25/08/11
Abstract |
Rationale |
The flow cytometry CD63 based basophil activation test (Basotest®) has already been validated for the diagnosis of immediate-type allergy such as venom, house dust mite or cypress pollen allergies. The aim of this study was to evaluate the interest of Basotest® for the diagnosis of natural rubber latex allergy.
Methods |
We included 46 latex allergic patients (who presented clinical symptoms of latex allergy, positive latex skin prick tests and specific IgE) and 33 control subjects and performed Basotest® in all subjects.
Results |
The sensitivity and specificity of Basotest® were 84.8% and 87.9% respectively when we considered the cut-off at 15% of CD63 positive cells. Using ROC curves, the optimal cut-off was evaluated at 22%, for which sensitivity and specificity were 79.3% and 96.7% respectively.
Conclusion |
The Basotest® is a reliable test in complement to clinical history and already validated tests (such as skin prick tests and specific IgE) to confirm the diagnosis of natural rubber latex allergy.
Le texte complet de cet article est disponible en PDF. | Funding: INSERM |
Vol 113 - N° 2S
P. S78 - février 2004 Retour au numéro
Article précédent
- Quality of patient reported outcome data captured using paper and interactive voice response diaries in an allergic rhinitis study; is electronic data capture really better?
- K. Weiler, A. Christ, G. Woodworth, R. Weiler, J.M. Weiler
- Life quality in patients presenting allergic rhinitis
- M.A. Calvo
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