Cross-linking of peanut allergens by polyphenol oxidase and its effect on peanut allergenicity - 25/08/11
Abstract |
Rationale |
Polyphenol oxidase (PPO) catalyzes the cross-linking of proteins. The objective was to determine if PPO induces cross-linking of peanut allergens and reduces peanut allergenicity.
Methods |
Protein extracts were prepared from defatted roasted peanut meals. The extracts were then incubated with PPO at 37 C, pH 7, for 60 min, and analyzed by SDS-PAGE, blots and competitive ELISA, using a pooled serum from peanut allergic individuals.
Results |
PPO reacted with two peanut proteins only. These were the major allergens Ara h 1 and Ara h 2. After PPO treatment, Ara h 1 and Ara h 2 were each seen to decrease significantly in levels. High molecular-weight polymers were formed due to the cross-linking of the allergens induced by PPO. IgE binding of the POD-treated peanut extracts was reduced approximately 4-fold as compared to the untreated.
Conclusions |
A significant decrease in the levels of two major peanut allergens Ara h 1 and Ara h 2 was observed after PPO treatment. Cross-linking of the allergens contributed to this decrease. A 4-fold reduction in IgE binding was observed. This suggests that PPO is capable of inducing a small reduction in peanut allergenicity.
Le texte complet de cet article est disponible en PDF. | Funding: Government |
Vol 113 - N° 2S
P. S314-S315 - février 2004 Retour au numéro
Article précédent
- Management of the multiple drug allergy syndrome - case report
- P.C.C. Pinto, L.M. Borrego, C.N. Braga, T. Almeida, J.E. Rosado-Pinto
- Validity of the diagnostic tests in egg allergy
- M.C. Diéguez, M. Antón, E. Camacho, D. Prieto, I. Herranz, M. Cuevas, B. De La Hoz
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