Serum IgE responses to Alternaria in Th1-biased Fisher-344 rats - 25/08/11
Abstract |
Rationale |
Allergens containing proteases can induce Th2 responses in Th1-biased mice, including airway hyperresponsiveness, BAL eosinophilia, peribronchial inflammation, and airway goblet cell metaplasia. We hypothesized that exposing Th1-biased rats to protease-containing allergen (crude Alternaria) would result in a Th2-biased response (increase in crude Alternaria-specific IgE levels).
Methods |
Using Fisher-344 rats, we evaluated IgE responses to crude Alternaria exposure. Group 1 was sensitized subcutaneously to crude Alternaria in AlOH3 at week 0 and exposed to nebulized crude Alternaria (150 mg protein) twice weekly for 5 weeks. Group 2 was sensitized subcutaneously to crude Alternaria in AlOH3 at week 0 and exposed to intranasal crude Alternaria (0.6 mg protein) twice weekly for 5 weeks. Group 3 (not sensitized) was exposed to nebulized crude Alternaria (150 mg protein) twice weekly for 5 weeks. Group 4 (not sensitized) was exposed to nebulized saline twice weekly for 5 weeks. Blood samples were drawn weekly. Crude Alternaria-specific IgE levels were evaluated via ELISA.
Results |
No statistically significant differences in crude Alternaria-specific IgE levels were demonstrated between groups (p>0.05). Protease content in the crude Alternaria extract (quantitated using a protease assay kit) was 130 ng/mg. Endotoxin level in the crude Alternaria extract (quantitated using an LAL test performed by Cambrex BioScience) was 21.5 EU/mg.
Conclusions |
We were unable to induce IgE responses to Alternaria in the Fisher-344 rat model. Neither the threshold dose of protease to induce Th2 responses or the threshold dose of endotoxin to induce Th1 responses in rodents are currently known.
Le texte complet de cet article est disponible en PDF. Funding: US Department of Veterans Affairs |
Vol 113 - N° 2S
P. S209 - février 2004 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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