Allergen specific IgG memory B cells in atopic and non-atopic individuals - 25/08/11
Abstract |
Rationale |
Clinical manifestations of type 1 hyperreactivity are triggered when allergens cross-link effector cell-bound IgE and aggregate the underlying high affinity FcϵRI. While the IgE antibody-mediated release of mediators constitutes the immunopathological basis for immediate symptoms, much less is known concerning the role of allergen-specific responses of isotypes other than IgE.
Methods |
Our analysis of the major birch pollen (Betv1) specific immune response showed for the first time that Betv1 specific memory B cells are present in tonsils and peripheral blood of both atopic and non-atopic individuals.
Results |
RT-PCR of single Betv1 specific B cells revealed highly mutated sequences mainly of the IgG and IgA isotype. Striking was the high percentage of VH3-30 or VL3l gene rearrangements. This result was confirmed by an in vitro antibody affinity selection via phage panning of a human VH/VL phage library (Griffin.1) using recombinant Betv1. Recombinant antibodies with VH3-30 or VL3l rearrangement had the highest relative affinities as measured by plasmon resonance technology.
Conclusions |
As Betv1 plasma cells were detectable in tonsils only in atopic individuals, the question rises, how the differentiation of Betv1 specific memory B cells in non-atopic individuals is controlled and why Betv1 specific IgE antibodies as in case of atopic disease are not synthesized.
Le texte complet de cet article est disponible en PDF.![]() | Funding: J 2107, Austrian Science Fundation |
Vol 113 - N° 2S
P. S162 - février 2004 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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