Pure mite bodies generate high quality allergenic extracts - 25/08/11

Doi : 10.1016/j.jaci.2003.12.497

V. Mahakittikun ^a, C. Bunnag ^a, P. Vichyanond ^a, S. Wongkamchai ^a, C. Komoltri ^a, P. Eakpo ^a, J.J. Boitano ^b
^a Mahidol University, Bangkok, Thailand
^b Mahidol University, Stratford, CT, USA

Abstract

Rationale

The factors involving the quality and the efficacy of the mite allergenic extracts include the source of the raw material (the mite culture) and the conditions of storage. In this study, pure mite bodies (PMB) and spent mite medium (SMM) were extracted from Dermatophagoides pteronyssinus, and D. farinae culture to evaluate the best part used for the manufacture of mite allergenic extracts and to evaluate the effects of temperature, the glycerine preservative used for storage, and the stability of the extracts over the short and long term, as well as day-time clinic use.

Methods

The extract were carried out by means of SDS-PAGE and ELISA.

Results

The PMB yielded a significantly greater concentration of major allergens than the SMM, and displayed a prominent mite allergen profile. The stability of the extract decreased if it was exposed to room temperature for 3 hours per day over consecutive days for one year. If the extracts were maintained at room temperature, the major allergens exhibited a significant 2- to 3-fold decrease in concentration. As regards Der p, the extract with glycerine only decreased 2-fold whereas the extract without glycerine, decreased 3-fold (p-value < .05).

Conclusion

PMB rather than SMM should be used as a raw material source for the production of extracts of mite allergens and temperature has an effect on the stability and the concentration of mite allergens. Either glycerine- and non-glycerine-treated extracts may be kept for at least one year at 4 °C with significant changes in composition or concentration.

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