Quantification of fetomaternal hemorrhage: a comparative study of the manual and automated microscopic Kleihauer-Betke tests and flow cytometry in clinical samples - 25/08/11
, Wilma E. Mesker b, Godelieve M. de Groot-Swings a, Geeske G. Brouwer-Mandema c, Hans J. Tanke, PhD b, Humphrey H. Kanhai, MD, PhD a
Reprint requests: S.A. Scherjon, MD, PhD, Department of Obstetrics, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands.Abstract |
Objective |
The purpose of this study was to evaluate the quantification of fetomaternal hemorrhage by the manual and automated microscopic analysis of Kleihauer-Betke stained slides and by flow cytometry.
Study design |
Blood smears were stained and evaluated manually according to the Kleihauer-Betke test. The same slides were used for automated microscopy. In addition, blood flow cytometry was performed by anti–hemaglobin F immunostaining.
Results |
Fetomaternal hemorrhage >0.1% was detected in 4 patients by manual and automated Kleihauer-Betke test and by blood flow cytometry. Fetomaternal hemorrhage was absent according to all 3 methods in 13 patients; fetomaternal hemorrhage<0.1% was detected in 27 patients by either manual or automated Kleihauer-Betke test or both. Moderate agreement was observed between the manual and automated Kleihauer-Betke test (weighted κ, 0.56; 95% CI, 0.33–0.78). Agreement between the manual Kleihauer-Betke test and blood flow cytometry was fair (weighted κ, 0.40; 95% CI, 0.15–0.66).
Conclusion |
Automated microscopic detection of fetal blood cells in clinical samples provides accurate quantification that is comparable to the manual Kleihauer-Betke test in both small and large fetomaternal hemorrhage. Blood flow cytometry is capable only of quantifying fetomaternal hemorrhage of >0.1%.
Le texte complet de cet article est disponible en PDF.Keywords : Fetomaternal hemorrhage, Fetal blood cell, Kleihauer-Betke test, Automated microscopy, Flow cytometry
Plan
Vol 191 - N° 2
P. 551-557 - août 2004 Retour au numéro
Article précédent
- Screening for post-miscarriage psychiatric morbidity
- Ingrid H. Lok, Dominic T.S. Lee, Shing-Kai Yip, Daniel Shek, Wing H. Tam, Tony K.H. Chung
- Maternal-fetal transfer and amniotic fluid accumulation of protease inhibitors in pregnant women who are infected with human immunodeficiency virus
- Hélène Chappuy, Jean-Marc Tréluyer, Elisabeth Rey, Jérôme Dimet, Maria Fouché, Ghislaine Firtion, Gérard Pons, Laurent Mandelbrot
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?