TGF-β differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release by human airway smooth muscle cells - 25/08/11
, Pieter S. Hiemstra, PhD, Klaus F. Rabe, MD, PhD
Reprint requests: Suzanne Zuyderduyn, MSc, Department of Pulmonology, C3-P, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands.Leiden, The Netherlands
Abstract |
Background |
Human airway smooth muscle cells (HASMs) are involved in the pathogenesis of asthma. By producing chemokines, HASMs play a role in the inflammatory processes observed in this disease. Eotaxin, eotaxin-2, and eotaxin-3 are important chemoattractants for eosinophils, and these chemokines are expressed during different phases of the allergic reaction. TH2 cytokines and TGF-β can be found in increased levels in patients with asthma, and these cytokines may be involved in the regulation of chemokine expression.
Objective |
The aim of this study was to determine the effect of TH2 cytokines and TGF-β on the regulation of expression of eotaxin, eotaxin-2, and eotaxin-3 by HASMs.
Methods |
HASMs were incubated for 24 hours with IL-4, IL-13, TGF-β1, or combinations of these cytokines. Protein and mRNA levels of eotaxin and eotaxin-3 were evaluated by sandwich ELISA and reverse transcriptase-PCR.
Results |
IL-4 and IL-13 induced mRNA and protein for both eotaxin and eotaxin-3. Eotaxin-2 mRNA and protein were not detected in HASMs. TGF-β alone did not induce expression of the eotaxins. However, in combination with IL-4 or IL-13, TGF-β enhanced eotaxin production and inhibited TH2 cytokine-induced eotaxin-3 production.
Conclusion |
TGF-β differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release.
Le texte complet de cet article est disponible en PDF.Key words : Human airway smooth muscle cells, TH2 cytokines, TGF-β, chemokines, allergy, asthma
Abbreviations used : BAL, CCL, HASM, MCP, MMP
Plan
| Funded by The Netherlands Asthma Foundation (grant 00.17) and AstraZeneca (Lund, Sweden). |
Vol 114 - N° 4
P. 791-798 - octobre 2004 Retour au numéro
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