Gene expression profiles in human nasal polyp tissues studied by means of DNA microarray - 25/08/11
, Jean Kim, MD, PhD a, , Joseph P. Sypek, PhD b, I-Ming Wang, PhD b, Heidi Horton b, Frank G. Oppenheim, DMD, PhD c, Bruce S. Bochner, MD a, ⁎ 
Reprint requests: Bruce S. Bochner, MD, Division of Allergy and Clinical Immunology, Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle, Rm 2B.71, Baltimore, MD 21224-6821.Baltimore, Md, and Cambridge and Boston, Mass
Résumé |
Background |
Nasal polyposis (NP) is a chronic inflammatory disease of the sinuses. Its pathogenesis is unknown. DNA microarray analysis allows simultaneous measurement of expression of thousands of genes in the same tissue sample and might help to identify gene alterations in various disorders.
Objective |
We sought to screen for disease-related genes in NP by using DNA microarrays and to validate the altered expression of selected genes at the mRNA and protein level.
Methods |
Expression microarrays containing approximately 10,500 genes were used to compare individual gene profiles of NP samples (n=10) and normal mucosal samples obtained from sphenoid sinuses in patients undergoing pituitary surgery (n=4). Four of the 5 most upregulated, and the single most downregulated, genes were retested by means of quantitative RT-PCR and immunohistochemistry in a different set of NP and normal mucosal samples obtained from the ethmoid and sphenoid sinuses.
Results |
Compared with normal sinus tissue, 192 genes were upregulated at least 2-fold, and 156 genes were downregulated by at least 50% in NP samples (approximately 3% of genes evaluated). Four of the top 5 overexpressed genes (statherin, 48.0-fold; prolactin-induced protein [PIP], 24.9-fold; lactoferrin, 26.6-fold; and deleted in malignant brain tumor 1 [DMBT1], 30.3-fold) and the most underexpressed gene (Clara cell 10-kd protein [CC10], −20.1-fold) were selected and retested by means of quantitative RT-PCR and immunohistochemical staining. Quantitative RT-PCR and immunohistochemical staining confirmed the differential expression of all except statherin in NP tissue.
Conclusion |
DNA microarrays can provide new insight into the possible pathophysiologic processes involved in NP.
Le texte complet de cet article est disponible en PDF.Key words : Nasal polyp, sinus, DNA microarray, gene expression, statherin, prolactin-induced protein, lactoferrin, deleted in malignant brain tumor 1, Clara cell 10-kd protein
Abbreviations used : CC10, CRS, DMBT1, LP, NP, PAS, PIP, SRCR
Plan
| Dr Liu is currently affiliated with the Department of ENT, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. Dr Wang is currently affiliated with Merck Research Laboratory, Molecular Profiling, Seattle, Wash. Supported by NIH/NIDCR grants DE07652, DE05672, and DE14950 (to F.G.O.). |
Vol 114 - N° 4
P. 783-790 - octobre 2004 Retour au numéro
Article précédent
- Inducible costimulator–positive T cells are required for allergen-induced local B-cell infiltration and antigen-specific IgE production in lung tissue
- Katja C. Beier, Andreas Hutloff, Max Löhning, Tilmann Kallinich, Richard A. Kroczek, Eckard Hamelmann
- TGF-β differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release by human airway smooth muscle cells
- Suzanne Zuyderduyn, Pieter S. Hiemstra, Klaus F. Rabe
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?