Tissue transglutaminase autoantibody detection in human saliva: a powerful method for celiac disease screening - 24/08/11
, Mirella Ferri, MD, Raffaella Nenna, MD, Antonella Verrienti, BSc, Umberto Di Mario, MD, PhD, Claudio Tiberti, BScs
Reprint requests: Prof. Margherita Bonamico, Istituto di Clinica Pediatrica, Policlinico “Umberto I,” Viale Regina Elena 324, 00161 Rome, Italy.Abstract |
Objective |
To test the possibility of detecting tissue transglutaminase autoantibodies (tTG-Abs) in saliva with a novel sensitive fluid-phase radioimmunoassay (RIA).
Study design |
Paired saliva and serum samples from 39 patients with celiac disease (CD), at the first biopsy (Group 1: 28 females, mean age 11.5 ± 11.1 years); 32 controls with a normal duodenal mucosa (Group 2: 18 females, mean age 8.1 ± 3.6 years); and 32 healthy volunteers (Group 3: 21 females, mean age 31.7 ± 9.8 years) were studied for tTG-Ab presence. Limit of positivity for salivary assay was calculated according to the 99th percentiles of Group 2 control children and was expressed as an autoantibody (Ab) index.
Results |
Salivary tTG-Abs were found in 97.4% of the patients with CD and in 100% of the corresponding serum samples. All Group 3 subjects were negative with both saliva and serum assays. A correlation between saliva and serum tTG-Ab titers was found (r=0.826, P=.0014).
Conclusions |
This study demonstrates that it is possible to detect salivary tTG-Abs in CD with a non-invasive, simple to perform, reproducible and sensitive method.
Le texte complet de cet article est disponible en PDF.Abbreviations : Ab, AGA, CD, EMA, IgA, RIA, ROC, tTG-Abs
Plan
 | Supported in part by a grant from the Ministero dell'Università, dell'Istruzione e della Ricerca (MUIR) and by a grant from the Ministero della Sanità. |
Vol 144 - N° 5
P. 632-636 - mai 2004 Retour au numéro
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