Leukotrienes and 8-isoprostane in exhaled breath condensate of children with stable and unstable asthma - 24/08/11
Reprint requests: Dr Eugenio Baraldi, Department of Pediatrics, Via Giu-stiniani 3, 35128 Padova, ItalyAbstract |
Background |
Cysteinyl-leukotrienes (cys-LTs) and 8-isoprostane are biomarkers of airway inflammation and oxidative stress.
Objective |
The aim of this study was to evaluate cys-LT and 8-isoprostane levels in exhaled breath condensate (EBC) of children with different degrees of asthma severity.
Methods |
EBC was collected from 14 steroid-naive children with mild persistent asthma, 13 children with stable mild- to-moderate persistent asthma treated with inhaled corticosteroids (ICS), 9 ICS-treated children with unstable asthma, and 19 healthy children.
Results |
In the three groups of asthmatic children, EBC concentrations of cys-LTs and 8-isoprostane were significantly higher than in control children (steroid-naive asthmatic children: cys-LTs median, 10.8 pg/mL, P < .001, 8-isoprostane, 16.2 pg/mL, P < .001; ICS-treated stable asthmatic children: cys-LTs, 12.7 pg/mL, P < .001, 8-isoprostane, 18.1 pg/mL, P < .001; children with unstable asthma: cys-LTs, 106.0 pg/mL, P < .01, 8-isoprostane, 29.7 pg/mL, P < .01; control children: cys-LTs, 4.3 pg/mL, 8-isoprostane, 3.5 pg/mL). Cys-LT levels were higher in children with unstable asthma than in the other two asthmatic groups (P < .05). FENO levels were significantly higher in steroid-naive and in children with unstable asthma compared with ICS-treated children with stable asthma (P < .01).
Conclusions |
Our study shows that EBC cys-LTs and 8-isoprostane concentrations are higher in asthmatic children than in healthy control children, with scattered values in patients with unstable asthma. These findings suggest that EBC eicosanoid measurement may have useful clinical implications for investigating phenotype differences among asthmatic patients.
Le texte complet de cet article est disponible en PDF.Keywords : Asthma, children, exhaled breath condensate, cysteinyl-leukotrienes, 8-isoprostane, exhaled nitric oxide, oxidative stress
Abbreviations : EBC, Cys-LTs, FENO, ICS, IQR
Plan
 | Supported in part by a grant of Regione Veneto, Ricerche Sanitarie Finalizzate (No. 885/03/99). |
Vol 113 - N° 2
P. 257-263 - février 2004 Retour au numéro
Article précédent
- Both inflammation and remodeling influence nitric oxide output in children with refractory asthma
- Bruno Mahut, Christophe Delclaux, Isabelle Tillie-Leblond, Philippe Gosset, Christophe Delacourt, Françoise Zerah-Lancner, Alain Harf, Jacques de Blic
- Asthma deaths during sports : Report of a 7-year experience
- Jack M Becker, James Rogers, Gregory Rossini, Haresh Mirchandani, Gilbert E D'Alonzo
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?