Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil - 23/08/11

Doi : 10.1016/j.tube.2008.06.002

João Alves de Araújo-Filho ^a,^b, Arioldo Carvalho Vasconcelos ^a, Eduardo Martins de Sousa ^a, André Kipnis ^a, Elisângela Ribeiro ^c, Ana Paula Junqueira-Kipnis ^a,^⁎
^a Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua Delenda Rezende de Melo, S/N^o, Setor Universitário, Goiânia, GO 74605-050, Brazil
^b Hospital de Doenças Tropicais Dr. Anuar Auad, Secretaria de Saúde do Estado de Goiás, Alameda Contorno, N^o 3556, Jardim Bela Vista, Goiânia, GO 74853-120, Brazil
^c Hospital Araújo Jorge, Associação de Combate ao Câncer em Goiás, Rua 239, No. 181, Setor Universitário, Goiânia, GO 74605-070, Brazil

Corresponding author. Tel.: +55 62 3209 6126; fax: +55 62 3521 1839.

Summary

Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis-specific CD8⁺ and CD4⁺ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4⁺IFN-γ⁺, CD4⁺IL-10⁺, CD8⁺IFN-γ⁺ and CD8⁺IL-10⁺ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-γ and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8⁺IFN-γ⁺ responses to ESAT-6 and CD8⁺IL-10⁺ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-γ and IL-10 responses and increased/normal specific CD8 IFN-γ and IL-10 responses. This suggests an important role for CD8 function in these patients.

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Keywords : Multi-drug resistant tuberculosis, Interferon-γ, Interleukin-10, MPT-51, GlcB

Plan

Introduction

Materials and methods

Participants groups

Preparation and stimulation of peripheral blood mononuclear cells (PBMC)

Flow cytometry

Statistical analysis

Results

Clinical, radiological and laboratorial characteristics of study patients

Specific CD4⁺IFN-γ⁺ and CD8⁺IFN-γ⁺cell response to recombinant MPT-51, GlcB and ESAT-6 from M. tuberculosis

Specific CD4⁺IL-10⁺ and CD8⁺IL-10⁺cell responses to recombinant MPT-51, GlcB and ESAT-6 from M. tuberculosis

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Vol 88 - N° 5

P. 474-481 - septembre 2008 Retour au numéro

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Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil - 23/08/11

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