Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China - 23/08/11

Doi : 10.1016/j.tube.2008.02.002

Rendong Fang ^a,^b,^d, Xia Li ^b, Jing Li ^a, Jie Wu ^a, Xin Shen ^a, Xiaohong Gui ^a, Kathryn DeRiemer ^c, Li Liu ^d, Jian Mei ^a,^⁎ , Qian Gao ^b,^⁎
^a Shanghai Institutes of Preventive Medicine, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
^b Key Laboratory of Medical Molecular Virology, Institute of Biomedical Sciences, Institute of Medical Microbiology, Fudan University, Shanghai 200032, China
^c School of Medicine, University of California, One Shields Avenue, Davis, CA 95616, USA
^d Department of Veterinary Medicine, College of Animal Science and Technology, Southwest University, Chongqing 400716, China

Corresponding author at: Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai 200032, China, Tel.: +86 21 5423 7195; fax: +86 21 5423 7971.Corresponding author. Tel./fax: +86 21 6278 1851.

Summary

We applied a 7 loci Variable-Number-Tandem-Repeats (VNTR-7) analysis method to identify mixed infections of Mycobacterium tuberculosis and to estimate the rate of mixed infections among pulmonary tuberculosis patients in Shanghai, China. We validated the VNTR-7 method and used it to genotype an isolate from each of the 249 from pulmonary tuberculosis patients reported from the Songjiang and Chongming districts in Shanghai during 2006. We identified 14 patients with mixed infections, and the estimated rate of mixed infections was 5.6% (14/249) (95% CI 3.1%–9.2%). Mixed infections were observed more frequently among tuberculosis patients undergoing retreatment (15.6%) than among new cases (4.1%) (p<0.05), and among tuberculosis patients whose disease was caused by non-Beijing genotype strains (12.5%) versus Beijing genotype strains (3.5%) (p<0.05). The VNTR-7 method is a highly sensitive, practical tool with relatively high discriminatory power, making it useful for studying mixed infections.

Le texte complet de cet article est disponible en PDF.

Keywords : Tuberculosis, Mixed infections, VNTR

Plan

Introduction

Materials and methods

Patients and isolates

Drug susceptibility testing (DST)

VNTR genotyping

Deletion-Targeted Multiplex-PCR (DTM-PCR) genotyping

Definition of mixed infections

Steps to minimize laboratory cross contamination

Statistical methods

Results

Patients and isolates

Evaluation of the VNTR genotyping method

Rate of mixed infections among pulmonary tuberculosis patients

Beijing genotype strains and mixed infections

Discussion

Export

Vol 88 - N° 5

P. 469-473 - septembre 2008 Retour au numéro

Article précédent

Resuscitation-promoting factors are expressed in Mycobacterium tuberculosis-infected human tissue
A.P. Davies, A.P. Dhillon, M. Young, B. Henderson, T.D. McHugh, S.H. Gillespie

| Article suivant

Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil
João Alves de Araújo-Filho, Arioldo Carvalho Vasconcelos, Eduardo Martins de Sousa, André Kipnis, Elisângela Ribeiro, Ana Paula Junqueira-Kipnis

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

Mixed infections of Mycobacterium tuberculosis in tuberculosis patients in Shanghai, China - 23/08/11

Summary

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL