Morphometric analysis of Th₁ and Th₂ cytokine expression in human pulmonary tuberculosis - 22/08/11

Doi : 10.1016/j.tube.2004.05.001

Xiyuan Bai ^a, Sarah E. Wilson ^a, Kathryn Chmura ^a, Nicole E. Feldman ^a, Edward D. Chan ^a,^b,^c,^d,⁎, ^{^{1
Supported by the American Lung Association Career Investigator Award, Bettina Garthwaite Lowerre Foundation for Mycobacteriology Research Fellowship, and NIH-HL-66-112.}}
^a Department of Medicine, University of Colorado School of Medicine, Denver, CO 80206, USA
^b Division of Pulmonary Sciences and Critical Care Medicine, University of Colorado School of Medicine, Denver, CO 80206, USA
^c Program in Cell Biology, National Jewish Medical and Research Center, University of Colorado School of Medicine, Denver, CO 80206, USA
^d Denver Veterans Administration Medical Center, University of Colorado School of Medicine, Denver, CO 80206, USA

^*Corresponding author. K613e, Goodman Building, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA. Tel.: +1-303-398-1491; fax: +1-303-398-1806.

Summary

Setting: Following infection with Mycobacterium tuberculosis, host cytokine responses influence disease manifestation. Differences in cytokine expression likely determine whether tuberculosis (TB) progresses, resolves, or becomes latent. In particular, the balance between Th₁ and Th₂ cytokine responses influences the expression of disease in individuals with pulmonary TB.

Objective and Design: Since the cytokine microenvironment in pulmonary TB remains suboptimally defined, we utilized quantitative immunohistochemistry to compare the expression of Th₁ cytokines [interferon-gamma (IFNγ) and interleukin-12 (IL-12)] and Th₂ cytokines [IL-4, IL-10, transforming growth factor-beta (TGFβ)] in surgically resected lungs of seven TB patients and four control subjects. We also quantified IFNγ-inducible protein 10 (IP-10) expression, a CXC chemokine for macrophages and T cells.

Results: Morphometric analyses revealed increased IFNγ, IL-12, IP-10, and TGFβ in granulomas and in pneumonitis areas of TB lungs. In contrast, IL-10 and IL-4 expressions were globally reduced in TB lung tissues compared to controls.

Conclusion: Th₁ cytokines and TGFβ are increased while Th₂ cytokines are decreased in well-formed pulmonary granulomas of TB patients compared to controls.

Le texte complet de cet article est disponible en PDF.

Keywords : Mycobacterium Tuberculosis, IP-10, IFNγ, IL-12, TGFβ, IL-10, IL-4, Immunohistochemistry

Plan

Introduction

Experimental Procedure

Materials

Immunohistochemical staining

Morphometric analysis

Isolation and differentiation of human monocyte-derived macrophages

Statistical analysis

Results

Expression of IFNγ and IL-12

Expression of IP-10

Expression of TGF-β, IL-4, and IL-10

Localization of macrophages, T-cells, and B-cells

Discussion

Export

Vol 84 - N° 6

P. 375-385 - 2004 Retour au numéro

Article précédent

Pharmacokinetics and relative bioavailability of clofazimine in relation to food, orange juice and antacid
David E. Nix, Rodney D. Adam, Barbara Auclair, Todd S. Krueger, Paul G. Godo, Charles A. Peloquin

| Article suivant

A matrix metalloproteinase inhibitor promotes granuloma formation during the early phase of Mycobacterium tuberculosis pulmonary infection
A.A. Izzo, L.S. Izzo, J. Kasimos, S. Majka

Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.

Déjà abonné à cette revue ?

connectez-vous ou créez un compte

Morphometric analysis of Th₁ and Th₂ cytokine expression in human pulmonary tuberculosis - 22/08/11

Summary

Plan

Export citations

Fichier

Contenu

Accès rapides

Mon compte

Aide & support

Plateformes Elsevier Masson

Déclaration CNIL