Fortifying Fresh Human Milk with Commercial Powdered Human Milk Fortifiers Does Not Affect Bacterial Growth During 6 Hours at Room Temperature - 21/08/11
Abstract |
Objective |
To evaluate the growth of resident aerobic mesophilic flora and added Enterobacter sakazakii in fresh, unfortified human milk; fresh human milk fortified with two commercial powdered fortifiers differing in iron content; and infant formula prepared from powder.
Subjects |
Eight mothers provided preterm breast milk samples.
Methods |
Breast milk samples were divided into three aliquots: unfortified, fortified with fortifier containing 1.44 mg iron/14 kcal, and fortified with fortifier containing 0.4 mg iron/14 kcal. Aliquots of formula were prepared. Breast milk and formula aliquots were divided into two test samples. Half were inoculated with low amounts of E sakazakii; half were not. All test samples were maintained at room temperature (22°C), serially diluted, and plated onto agars after 0, 2, 4, and 6 hours. Plates were incubated at 35°C and enumerated.
Statistical analyses |
Data were analyzed using repeated measures analysis of variance. P<.05 was considered significant.
Results |
There were no differences in colony counts of aerobic bacteria among uninoculated or among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. There were no differences in colony counts of E sakazakii among inoculated human milk samples at any time; counts did not increase significantly over 6 hours. Aerobic bacteria and E sakazakii colony counts from infant formula did not increase significantly over 6 hours.
Conclusions |
During 6 hours at 22°C, fresh human milk and formula had negligible bacterial growth; fortifying human milk with powdered fortifiers did not affect bacterial growth.
Le texte complet de cet article est disponible en PDF.Plan
Vol 105 - N° 10
P. 1567-1572 - octobre 2005 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?