Cabbage lipid transfer protein Bra o 3 is a major allergen responsible for cross-reactivity between plant foods and pollens - 20/08/11
Reprint requests: Carlos Blanco, MD, PhD, Servicio de Alergia, Hospital de Gran Canaria Dr Negrín, C/Bco de la Ballena s/n, 35012 Las Palmas de GC, Spain.Madrid and Las Palmas de Gran Canaria, Spain
Abstract |
Background |
Food IgE-mediated allergy to members of the Brassicaceae family has been increasingly reported.
Objective |
To characterize cabbage—Brassica oleracea var capitata—allergy and its major allergens.
Methods |
A prospective study was performed, recruiting 17 patients allergic to cabbage, and control subjects. Skin prick tests and double-blind placebo-controlled food challenges were performed. A major allergen was isolated from cabbage by RP-HPLC and characterized by N-terminal amino acid sequencing and matrix-assisted laser desorption/ionization mass spectrometry analysis. Specific IgE determinations, IgE immunoblots, and CAP-inhibition assays were also performed.
Results |
Skin prick test and specific IgE were positive to cabbage in all patients. Five of them referred anaphylactic reactions when eating cabbage, and in another 5 patients, cabbage allergy was further confirmed by double-blind placebo-controlled food challenge. Most of them showed associated sensitizations to mugwort pollen, mustard, and peach. A 9-kd cabbage IgE-binding protein, Bra o 3, was identified as a lipid transfer protein (LTP) with 50% of identity to peach LTP Pru p 3. Skin prick test with Bra o 3 showed positive results in 12 of 14 cases (86%). On CAP inhibition assays, Bra o 3 managed to inhibit significantly the IgE binding to cabbage, mugwort pollen, and peach. Both Bra o 3 and Pru p 3 were recognized by IgE from the patients’ sera.
Conclusion |
Bra o 3, a cabbage LTP, is a major allergen in this food, cross-reacting with mugwort pollen and with other plant foods, such as peach.
Clinical implications |
Cabbage IgE-mediated allergy is a potentially severe condition that can present with other plant food and pollen allergies.
Le texte complet de cet article est disponible en PDF.Key words : Allergen, anaphylaxis, Brassica oleracea, cabbage, cross-reactivity, food allergy, lipid transfer protein, mugwort pollen, mustard, peach
Abbreviations used : DBPCFC, LTP, MALDI, OAS, rPru p 3, SPT
Plan
| Supported by Instituto de Salud Carlos III (ISCIII-RTIC-G03/094 and RedRespira-ISCIII-RTIC-C03/011), and by Direccion General de Investigacion, Ministerio de Ciencia y Tecnología (project BMC 2002-00196). Disclosure of potential conflict of interest: The authors have declared they have no conflict of interest. |
Vol 117 - N° 6
P. 1423-1429 - juin 2006 Retour au numéro
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