Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1
0101 tetramer-binding CD4+ T cells
- 20/08/11
Reprint requests: Graham Stuart Ogg, FRCP, DPhil, MRC Human Immunology Unit, University of Oxford, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom.Oxford, United Kingdom
Abstract |
Background |
Although substantial evidence suggests that T cells are important in the pathogenesis of atopic dermatitis (AD), little is known of the differentiation status of CD4+ T cells specific for common environmental allergens.
Objective |
To determine the frequency, differentiation phenotype, and function of circulating allergen-specific CD4+ T cells in adult individuals with severe persistent AD and controls.
Methods |
Using tetrameric complexes of an HLA DRB10101 restricted epitope from Fel d 1, the major IgE-reactive component of cat dander, we studied ex vivo and cultured T-cell frequency and phenotype in individuals with AD and healthy controls. Cytokine secretion was measured by ex vivo and cultured IFN-γ, IL-4, and IL-10 enzyme linked immuno-spot analysis.
Results |
Ex vivo Fel d 1–specific DRB10101-restricted CD4+ T cells express high levels of CCR7, CD62L, CD27, and CD28 and proportionately low levels of tissue-specific homing receptors and TH1 and TH2 cytokine production, placing the cells largely within the central memory subgroup.
Conclusion |
Circulating Fel d 1-specific DRB10101-restricted CD4+ T cells maintain central memory capacity, consistent with a potential to contribute to persisting clinical atopic disease.
Clinical implications |
Persisting central memory characteristics of allergen-specific CD4+ T cells in individuals with AD may contribute to chronic disease.
Le texte complet de cet article est disponible en PDF.Key words : T cells, atopic dermatitis, memory T cells, Fel d 1
Abbreviations used : AD, CLA, DR1 LPV, ELISpot, R0, R10, TCM, TEM, UK
Plan
| Supported by the Medical Research Council and the Wellcome Trust. Disclosure of potential conflict of interest: G. S. Ogg and E. A. Bateman have received grant support from the Medical Research Council. M. R. Ardern-Jones has received grant support from Wellcome Trust. |
Vol 118 - N° 6
P. 1350-1356 - décembre 2006 Retour au numéro
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