Infection of cattle caused by Brucella abortus (ie, bovine brucellosis) has been of political importance in the United States for many decades. The most common clinical manifestation of brucellosis in natural hosts is reproductive loss resulting from abortion, birth of weak offspring, or infertility. Brucellosis regulatory programs were primarily developed as the most efficient way to prevent human infections. This article discusses cattle vaccination with B abortus strains 19 and RB51. Other reservoir hosts for this organism and other Brucella spp in cattle have also been visited.