Usefulness of flow cytometry in the diagnosis of mycosis fungoides - 19/08/11
Reprint requests: Howard J. Meyerson, MD, Department of Pathology, Case Western Reserve University/University Hospitals of Cleveland, 11100 Euclid Ave, Cleveland, OH 44106.Cleveland, Ohio
Abstract |
Background |
The pathologic evaluation of mycosis fungoides (MF) is a challenging area in dermatopathology.
Objective |
We sought to determine the usefulness of flow cytometry for the diagnosis of MF from skin biopsy specimens.
Methods |
Skin biopsy specimens from 22 patients with a clinical suggestion for MF were evaluated by 4-color flow cytometry. The results were correlated with the International Society for Cutaneous Lymphoma (ISCL) MF diagnostic score and molecular studies for T-cell receptor gene rearrangement.
Results |
A T-cell abnormality by flow cytometry was identified in all 11 patients with diagnostic ISCL scores whereas the 7 patients with either subdiagnostic ISCL scores or reactive histology showed no phenotypic abnormality by flow cytometry. In all, 10 of 11 patients with diagnostic skin biopsy specimens for MF had T-cell receptor gene rearrangements by polymerase chain reaction. Gene rearrangements were not detected in the subdiagnostic group.
Limitations |
Small study size was a limitation.
Conclusion |
Flow cytometry of skin biopsy specimens is a sensitive method for detecting abnormalities in MF and should be considered part of the routine workup of patients with a clinical suggestion of MF.
Le texte complet de cet article est disponible en PDF.Abbreviations used : CTCL, ISCL, MF, PCR, SS, TCR
Plan
| Supported by National Institutes of Health National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) AR 007569-15 (Drs Cooper and Apisarnthanarax), NIAMS Case Skin Disease Research Center P30-AR-39750 (Drs Cooper and Gilliam), and NIAMS RO1 AR 049284 (Dr Gilliam). Conflicts of interest: None declared. |
Vol 57 - N° 3
P. 454-462 - septembre 2007 Retour au numéro
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