Vascular endothelial growth factor modulates contractile response in microvascular lung pericytes - 19/08/11
: F.A.C.S., Robert F. Wilson, M.D : F.A.C.S.
Corresponding author. Tel.: +1-313-745-8771; fax: +1-313-745-2965Abstract |
Background |
Pericytes are capillary support cells that may play a role in regulating permeability by their contractile responses. Vascular endothelial growth factor (VEGF) may play a role in the increased permeability found in sepsis and other inflammatory conditions. The purpose of this study was to evaluate the role of VEGF in regulating pericyte contraction.
Methods |
Rat microvascular lung pericytes were isolated according to previously described methods and cultured on collagen gel matrices. Cells were exposed to VEGF (10, 100, and 1000 pg/mL) for varying time periods (0, 10, 30, 60, and 120 minutes). The gels were released and their contractile responses digitally quantified.
Results |
At all doses, VEGF induced initial pericyte relaxation (contraction 85% to 90% of controls; P < .001). This was followed-up by increased and sustained contraction (107% to 120% of controls; P < .01).
Conclusions |
VEGF modifies the contractile response of microvascular lung pericytes. This mechanism may play a role in the increased permeability demonstrated in inflammatory states.
Le texte complet de cet article est disponible en PDF.Keywords : Inflammation, Microvascular permeability, Pericyte, Sepsis, Vascular endothelial growth factor
Plan
Vol 191 - N° 3
P. 349-352 - mars 2006 Retour au numéro
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