Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats - 19/08/11
Reprints: Chandrasekhar Yallampalli, DVM, PhD, Distinguished Professor, Obstetrics and Gynecology, University of Texas-Medical Branch, 301 University Blvd., Galveston, TX 77555-1062Résumé |
Objective |
The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins.
Study Design |
Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE+D+ or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor NG-nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated.
Results |
Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE+D+>AfaE+D−>AfaE−D+) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment.
Conclusion |
These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa+ E coli gestational tropism.
Le texte complet de cet article est disponible en PDF.Key words : adhesive structure, Dr family, Escherichia coli, growth restriction, intrauterine infection, L-NAME, mortality rate, nitric oxide, rat
Plan
| Supported in part by National Institutes of Health Grants HL58144 and HL72620 (C.Y.), HD41687 (S.N.), and DK42029 (B.N.). |
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| Cite this article as: Wroblewska-Seniuk K, Nowicki S, Le Bouguénec C, et al. Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats. Am J Obstet Gynecol 2011;205:83.e1-7. |
Vol 205 - N° 1
P. 83.e1-83.e7 - juillet 2011 Retour au numéro
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