Photodynamic Actions of Indocyanine Green and Trypan Blue on Human Lens Epithelial Cells In Vitro - 18/08/11
, Neeru Kumar, MA, Saher M. Maswadi, PhD, Kenneth Zaslow, MD, Randolph D. Glickmank, PhD
Inquiries to Robert F. Melendez, MD, Univeristy of Texas Health Science Center at San Antonio, Department of Ophthalmology, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900; fax: (210) 567-8413Résumé |
Purpose |
The purpose of this study was to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lensepithelial cells (LECs).
Design |
Experimental study.
Methods |
Lens epithelial cell viability was assessed after treatment with ICG and TryB concentrations ranging from 0.025 to 5.0 mg/ml, and exposure to 806 nm diode laser.
Results |
At ICG concentrations below 0.5 mg/ml, there was ≥75% cell viability; at higher ICG concentrations there was dose-dependent cytotoxicity in addition to loss of cellular viability due to ICG photosensitization. TryB had little cytotoxicity to the LECs: >80% cells were viable irrespective of the dye concentration or laser treatment.
Conclusions |
These data indicate that ICG may have application as a photosensitizer in the selective eradication of residual LECs after cataract surgery to reduce the incidence of posterior capsule opacification.
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Vol 140 - N° 1
P. 132-134 - juillet 2005 Retour au numéro
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- Intraocular Pressure Over the Clinical Range of Blood Pressure: Blue Mountains Eye Study Findings
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- Dhananjay Shukla, Kannan B. Naresh, Ramasamy Kim
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