Photodynamic Actions of Indocyanine Green and Trypan Blue on Human Lens Epithelial Cells In Vitro - 18/08/11
Résumé |
Purpose |
The purpose of this study was to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lensepithelial cells (LECs).
Design |
Experimental study.
Methods |
Lens epithelial cell viability was assessed after treatment with ICG and TryB concentrations ranging from 0.025 to 5.0 mg/ml, and exposure to 806 nm diode laser.
Results |
At ICG concentrations below 0.5 mg/ml, there was ≥75% cell viability; at higher ICG concentrations there was dose-dependent cytotoxicity in addition to loss of cellular viability due to ICG photosensitization. TryB had little cytotoxicity to the LECs: >80% cells were viable irrespective of the dye concentration or laser treatment.
Conclusions |
These data indicate that ICG may have application as a photosensitizer in the selective eradication of residual LECs after cataract surgery to reduce the incidence of posterior capsule opacification.
Le texte complet de cet article est disponible en PDF.Vol 140 - N° 1
P. 132-134 - juillet 2005 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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