Optimization of Anterior Eye Fluorescein Viewing - 18/08/11
Résumé |
Purpose |
To optimize anterior eye fluorescein viewing and image capture.
Design |
Prospective experimental investigation.
Methods |
The spectral radiance of ten different models of slit-lamp blue luminance and the spectral transmission of three barrier filters were measured. Optimal clinical instillation of fluorescein was evaluated by a comparison of four different instillation methods of fluorescein into 10 subjects. Two methods used a floret, and two used minims of different concentration. The resulting fluorescence was evaluated for quenching effects and efficiency over time.
Results |
Spectral radiance of the blue illumination typically had an average peak at 460 nm. Comparison between three slit-lamps of the same model showed a similar spectral radiance distribution. Of the slit-lamps examined, 8.3% to 50.6% of the illumination output was optimized for >80% fluorescein excitation, and 1.2% to 23.5% of the illumination overlapped with that emitted by the fluorophore. The barrier filters had an average cut-off at 510 to 520 nm. Quenching was observed for all methods of fluorescein instillation. The moistened floret and the 1% minim reached a useful level of fluorescence in on average 20s (2.5× faster than the saturated floret and 2% minim) and this lasted for 160 seconds.
Conclusions |
Most slit-lamps’ blue light and yellow barrier filters are not optimal for fluorescein viewing and capture. Instillation of fluorescein using a moistened floret or 1% minim seems most clinically appropriate as lower quantities and concentrations of fluorescein improve the efficiency of clinical examination.
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Vol 142 - N° 4
P. 572 - octobre 2006 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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