Bovine tuberculosis in India: Potential basis for zoonosis - 17/08/11

Doi : 10.1016/j.tube.2005.08.005

H.K. Prasad ^a,^⁎ , A. Singhal ^a, A. Mishra ^a,^{^{1
Present address: Section of Experimental Haematology/Oncology, Department of Paediatric Haematology/Oncology, Medical School Hanover, Carl Neuberg Strasse 1, D-30625 Hanover, Germany.}} , N.P. Shah ^a, V.M. Katoch ^b, S.S. Thakral ^c, D.V. Singh ^c, S. Chumber ^d, S. Bal ^d, S. Aggarwal ^e, M.V. Padma ^f, S. Kumar ^g, M.K. Singh ^h, S.K. Acharya ⁱ
^a Department of Biotechnology, All India Institute of Medical Sciences, New Delhi-110029, India
^b Central JALMA Institute for Leprosy and other Mycobacterial Disease, Taj Ganj, Agra-282001, India
^c Central Military Veterinary Laboratory, Meerut Cantt-250001, India
^d Department of Surgery, All India Institute of Medical Sciences, New Delhi-110029, India
^e Department of Paediatric Surgery, All India Institute of Medical Sciences, New Delhi-110029, India
^f Department of Neurology, All India Institute of Medical Sciences, New Delhi-110029, India
^g Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi-110029, India
^h Department of Pathology, All India Institute of Medical Sciences, New Delhi-110029, India
ⁱ Department of Gastro-enterology, All India Institute of Medical Sciences, New Delhi-110029, India

Corresponding author. Tel.: +911126594994; fax: +911126852286, 26862435.

Summary

Our laboratory has designed a specific nested-PCR (N-PCR) assay, based on the hupB gene of Mycobacterium tuberculosis (Rv2986c) and Mycobacterium bovis (Mb3010c) as a method to differentiate these closely related species. The present paper deciphers the utility of this assay for identification of pathogenic Mycobacteria in clinical samples. Extra-pulmonary clinical samples obtained from cattle and humans were investigated. Pre-dominance of M. tuberculosis (15.7%) and M. bovis (26.8%) was seen in humans and cattle, respectively. However, more importantly, both mycobacterial pathogens (mixed infection) were identified in a number of samples. In humans 8.7% of the samples and 35.7% in cattle were classified as mixed infection. The detection of mixed infection with the mycoabcterial pathogenic duo in humans and bovines denotes the prospect of potential transmission of these pathogens from humans to cattle (zoonosis) and vice versa (reverse zoonosis).

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Keywords : Zoonotic TB, M. tuberculosis, M. bovis, Nested PCR, HupB