Quantitative analysis of residual protein contamination on reprocessed surgical instruments - 17/08/11
, H.C. Baxter, G.A. Campbell, K. Grant, A. Jones, P. Richardson, G. Whittaker
Corresponding author. Address: School of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK. Tel.: +44 131 6504708; fax: +44 131 6506453.Summary |
‘Ready-for-use’ instruments from surgical instrument trays were examined after routine cleaning and sterilization in a blinded study. These reprocessed instruments originated from five National Health Service hospital trust sterile service departments in England and Wales. Determination of residual protein and peptide contamination was carried out by acid stripping of the instrument surfaces, hydrolysis of the constituent amino acids and quantitative total amino acid analysis. One hundred and twenty instruments were analysed, and the median levels of residual protein contamination per instrument for the individual trays were 267, 260, 163, 456 and 756μg. Scanning electron microscopy and energy dispersive X-ray spectroscopic analyses of the instruments showed that tissue deposits were localized on surfaces, but there was no significant correlation between overall protein soiling and instrument complexity. The highest levels of residual contamination were found on instruments used for tonsillectomy and adenoid surgery.
Le texte complet de cet article est disponible en PDF.Keywords : Cleaning, Surgical instruments, Prion diseases, Sterile services, Protein determination
Plan
Vol 63 - N° 4
P. 439-444 - août 2006 Retour au numéro
Article précédent
- Surface decontamination of surgical instruments: an ongoing dilemma
- H. Murdoch, D. Taylor, J. Dickinson, J.T. Walker, D. Perrett, N.D.H. Raven, J.M. Sutton
- Potential for reported needlestick injury prevention among healthcare workers through safety device usage and improvement of guideline adherence: expert panel assessment
- B.L. Cullen, F. Genasi, I. Symington, J. Bagg, M. McCreaddie, A. Taylor, M. Henry, S.J. Hutchinson, D.J. Goldberg
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?