Validation of a Pseudomonas aeruginosa porcine model of septic shock - 16/08/11
, Abdulnasser Assadi b, Farida Benatir a, Emmanuel Boselli a, Catherine Kaminski a, Florence Arnal b, Corinne Lambert b, Joëlle Goudable b, Dominique Chassard a, Bernard Allaouchiche a
Corresponding author. Département d'Anesthésie-Réanimation, Pavillon P Réanimation, Hôpital Edouard Herriot, place d'Arsonval, 69003 Lyon, France. Tel.: +33 472 110 217; fax: +33 472 110 278.Summary |
Objectives |
To develop a standardized bacteraemic porcine model of septic shock with cardiovascular and immunological profiles similar to those observed in human clinical states.
Methods |
Sepsis was induced by an intravenous challenge of 18 anaesthetized pigs with live Pseudomonas aeruginosa. The pulmonary arterial pressure was monitored and the bacterial infusion was stopped when the systolic pulmonary arterial pressure reached 45mmHg. Septic shock was treated with fluid resuscitation and epinephrine infusion. The haemodynamic parameters and the rate of different inflammatory cytokines were recorded during 6h of observation.
Results |
The mean±SD cardiac output increased from 2.4±1.2 to 5.7±2.1L/min while the mean±SD systemic vascular resistance index decreased from 1957±744 to 709±221dyn/s/cm5/m2. The pharmacokinetic profile of the inflammatory cytokines was similar to the one observed in human studies.
Conclusions |
The control of the systolic pulmonary arterial pressure during a P. aeruginosa infusion leads to a hyperdynamic, reproducible cardiovascular profile similar to the one observed in human septic shock. Since the immunological profile of the inflammatory cytokines is also similar to the human one, this standardized porcine model appears to be appropriate for experimental research concerning sepsis.
Le texte complet de cet article est disponible en PDF.Keywords : Septic shock, Intensive care, Porcine model, Cytokines, Pseudomonas aeruginosa, Pig
Plan
Vol 53 - N° 3
P. 199-205 - septembre 2006 Retour au numéro
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