Mycobacteria chelonae was originally included in group IV of Runyon’s classification of atypical mycobacteria. This rapidly growing environmental organism often caused infections in immunocompromised hosts. Candida albicans is a ubiquitous micro-organism and frequently isolated from the gastrointestinal tract. These two micro-organisms seldom cause esophageal diseases in healthy humans. We report a case of a fruit farmer with a submucosa tumor of distal esophagus caused by coexistence of M. chelonae and C. albicans infections, which was successfully treated by surgical resection and following antimicrobial regimens postoperatively.