Recombinant guinea pig TNF-⍺ enhances antigen-specific type 1 T lymphocyte activation in guinea pig splenocytes - 15/08/11
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Summary |
TNF-⍺ is a principal pro-inflammatory cytokine which contributes to the activation of innate immunity and the transition to antigen-specific adaptive immunity in tuberculosis. Using recombinant guinea pig (rgp) TNF-⍺, the effect of TNF-⍺ on lymphocyte activation was examined in unvaccinated and BCG-vaccinated guinea pigs. Splenocytes were stimulated with PPD or ConA, in the presence or absence of rgp TNF-⍺ for 96h. Lymphocyte proliferation was measured using [3H]thymidine uptake, and IL-12 p40 and IFN-γ mRNA were analyzed using real-time PCR. rgpTNF-⍺ alone was able to stimulate a significant degree of proliferation in splenocytes. The addition of rgpTNF-⍺ to PPD-stimulated cells enhanced the proliferation of splenocytes from BCG-vaccinated guinea pigs. Furthermore, enhancement of proliferation by rgpTNF-⍺ was found to be correlated with upregulation of the levels of Type 1 cytokine mRNA (IL-12p40 and IFN-γ) in splenocyte cultures. This suggests that TNF-⍺ plays an important role in the regulation of Type 1 T cell-mediated immune responses in the guinea pig.
Le texte complet de cet article est disponible en PDF.Keywords : BCG, TNF-⍺ IFN-γ, Guinea pig
Plan
Vol 87 - N° 2
P. 87-93 - mars 2007 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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