TGFB1 promoter polymorphism C-509T and pathophysiology of asthma - 15/08/11
, Hisako Matsumoto, MD, PhD a, Masaya Takemura, MD, PhD a, Masafumi Yamaguchi, MD a, Hirofumi Matsuoka, MD a, Makiko Jinnai, MD a, Kazuo Chin, MD, PhD a, Masayoshi Minakuchi, MD, PhD b, Lei Cheng, MD, PhD c, Taro Shirakawa, MD, PhD d, Michiaki Mishima, MD, PhD a
Reprint requests: Akio Niimi, MD, PhD, Department of Respiratory Medicine, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, 606-8507, Japan.Abstract |
Background |
TGF-β1 can modulate airway inflammation and exaggerate airway remodeling. A polymorphism of a promoter region of TGFB1, C-509T, might be associated with the development of asthma, but its pathophysiologic relevance remains poorly understood.
Objective |
We investigated relations of the C-509T polymorphism to airflow obstruction, sputum eosinophilia, and airway wall thickening, as assessed by means of computed tomography, in 85 patients with stable asthma.
Methods |
The CC, CT, and TT genotypes were examined by means of PCR and restriction enzyme fragment length polymorphism. At a selected bronchus, 3 indices of airway wall thickness were measured with an automatic method.
Results |
The CC, CT, and TT genotypes were found in 22, 46, and 17 patients, respectively. Serum TGF-β1 levels were significantly associated with the polymorphism and were increased in the CT/TT genotypes. FEV1 and sputum eosinophil percentages were also significantly associated with the polymorphism and were both decreased in the CT/TT genotypes. The polymorphism was unrelated to airway wall thickness.
Conclusion |
In addition to increased serum TGF-β1 levels, the T allele of the C-509T polymorphism is related to increased airflow obstruction but attenuated eosinophilic inflammation. The former relation is not attributed to thickening of the central airway walls. The latter relation might reflect the anti-inflammatory effect of TGF-β1. The C-509T polymorphism has a complex role in asthma pathophysiology, presumably because of the diverse functions of TGF-β1 and its various interactions with cells and humoral factors in vivo.
Le texte complet de cet article est disponible en PDF.Key words : Asthma, airway remodeling, airway inflammation, TGF-β1, C-509T, gene polymorphism, computed tomography, airway wall thickening, sputum eosinophil
Abbreviations used : CT, WA
Plan
| Disclosure of potential conflict of interest: The authors have declared that they have no conflict of interest. |
Vol 121 - N° 3
P. 659-664 - mars 2008 Retour au numéro
Article précédent
- Increased activation-induced cell death of high IFN-γ–producing TH1 cells as a mechanism of TH2 predominance in atopic diseases
- Tunc Akkoc, Pieter J.A. de Koning, Beate Rückert, Isil Barlan, Mübeccel Akdis, Cezmi A. Akdis
- Reducing asthma health disparities in poor Puerto Rican children: The effectiveness of a culturally tailored family intervention
- Glorisa Canino, Doryliz Vila, Sharon-Lise T. Normand, Edna Acosta-Pérez, Rafael Ramírez, Pedro García, Cynthia Rand
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?