Low rate of occult hepatitis B virus infection among anti-HBc positive blood donors living in a low prevalence region in Brazil - 14/08/11

Doi : 10.1016/j.jinf.2004.07.007

C.M.D. Silva ^a,^b,⁎ , C. Costi ^a, C. Costa ^c, C. Michelon ^a, R. Oravec ^c,^d, A.B. Ramos ^e, C. Niel ^f, M.L. Rossetti ^a,^b
^a Center of Scientific and Tecnological Development, State Foundation for Productivity and Research for Public Health, Porto Alegre, Rio Grande do Sul, Brazil
^b Brazil Lutheran University, Canoas, Rio Grande do Sul, Brazil
^c Rio Grande do Sul Hemotherapy Center, State Foundation for Productivity and Research for Public Health, Porto Alegre, Rio Grande do Sul, Brazil
^d Weinmann Laboratory, Porto Alegre, Rio Grande do Sul, Brazil
^e Cardiology University Foundation, Alvorada, Rio Grande do Sul, Brazil
^f Department of Virology, Oswaldo Cruz Institute, Rio de Janeiro, Brazil

^*Corresponding author. Address: Centro de Desenvolvimento Científico e Tecnológico do Estado, Av. Ipiranga 5400, 90610-000 Porto Alegre, RS, Brazil. Tel./fax: +55-51-3352-0336.

Abstract

Objective

The aim of this study was to determine the rate of occult hepatitis B virus (HBV) infection among blood donors living in a geographic region of low (5.6%) anti-HBc prevalence.

Subjects and methods

Sera from 150 candidate blood donors whose blood was rejected due to total anti-HBc reactivity (despite absence of HBsAg) were tested for anti-HBs and IgM anti-HBc antibodies, as well as for HBeAg/anti-HBe. Serum HBV DNA was sought by using a PCR assay able to amplify part of the surface gene. Viral load was measured in the PCR positive samples.

Results

The pattern ’anti-HBc alone’ (without HBsAg and anti-HBs antibodies) was found in 64 (42.7%) subjects. IgM anti-HBc and anti-HBe antibodies were detected in 2 (1.3%) and 80 (53.3%) samples, respectively. No sample was HBeAg-reactive. HBV DNA was repeatedly found in five (3.3%) samples, three of which were anti-HBs positive and two anti-HBs negative. All five HBV DNA positive samples showed a low viral load (<1000copies/ml).

Conclusions

The data indicated a low rate of occult infection among anti-HBc positive, HBsAg negative blood donors living in a region of low prevalence of infection. Viral load was very low in all HBV infected subjects.

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Keywords : Blood donors, Hepatitis B virus, HBV DNA, Serological markers, Viral load

Plan

Introduction

Materials and methods

Samples

Serological studies

DNA extraction and PCR assays

Measurement of viral load

Results

Discussion

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Vol 51 - N° 1

P. 24-29 - juillet 2005 Retour au numéro

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Low rate of occult hepatitis B virus infection among anti-HBc positive blood donors living in a low prevalence region in Brazil - 14/08/11

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Subjects and methods

Results

Conclusions

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