Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan - 14/08/11

Doi : 10.1016/j.jinf.2004.12.015

Yayoi Otsuka ^a,^{^{1
Present address affiliation: Laboratory of Molecular Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan}}, Tomoko Fujino ^a, Namiko Mori ^a, Jun-ichiro Sekiguchi ^a, Emiko Toyota ^a, Katsutoshi Saruta ^a, Yoshihiro Kikuchi ^b, Yuka Sasaki ^c, Atsushi Ajisawa ^d, Yoshito Otsuka ^e, Hideaki Nagai ^f, Makoto Takahara ^g, Hideo Saka ^h, Takuma Shirasaka ⁱ, Yasuki Yamashita ⁱ, Makiko Kiyosuke ^j, Hideyuki Koga ^j, Shinichi Oka ^a, Satoshi Kimura ^a, Toru Mori ^k, Tadatoshi Kuratsuji ^a, Teruo Kirikae ^a,⁎
^a International Medical Center of Japan, Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, Japan
^b National Medical Organization, Sendai Medical Center, Miyagino 2-8-8, Miyagino-ku, Sendai 983-8520, Japan
^c National Hospital Organization, Chiba-Higashi Hospital, Nitona-cho 673, Chuo-ku, Chiba 260-8712, Japan
^d Tokyo Metropolitan Komagome Hospital, Honkomagome 3-18-22, Bunkyo-ku, Tokyo 113-8677, Japan
^e Social Insurance Central General Hospital, Hyakuninchou 3-22-1, Shinjuku-ku, Tokyo 169-0073, Japan
^f National Hospital Organization, Tokyo Hospital, Takeoka 3-1-1, Kiyose, Tokyo 204-8585, Japan
^g National Hospital Organization, West Kofu Hospital, Yamamiya 3368, Kofu 400-0075, Japan
^h National Hospital Organization, Nagoya Medical Center, Sannomaru 4-1-1, Naka-ku, Nagoya 406-0001, Japan
ⁱ National Hospital Organization, Osaka National Hospital, Hoenzaka 2-1-14, Chuo-ku, Osaka 540-0006, Japan
^j National Hospital Organization, Kyushu Medical Center, Jigyohama 1-8-1, Chuo-ku, Fukuoka 810-8563, Japan
^k Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Matsuyama 3-1-24, Kiyose, Tokyo 204-8533, Japan

^*Corresponding author. Tel.: +81 3 3202 7181x2838; fax: +81 3 3202 7364.

Abstract

Objective

To assess DNA polymorphisms in mycobacterial isolates obtained from human immunodeficiency virus (HIV)-seropositive patients with tuberculosis in Japan from 1996 to 2003.

Methods

Restriction fragment length polymorphisms (RFLP) from Mycobacterium tuberculosis and Mycobacterium avium isolates obtained from individual seropositive patients with tuberculosis (n=78) were analysed with the use of IS6110 and (CGG)₅ or IS1245 and IS1311, respectively, as markers. As a control, the same procedures were applied to isolates from HIV-seronegative tuberculosis patients (n=87).

Results

Of 86 mycobacterial strains, M. tuberculosis, M. avium and Mycobacterium chelonae were identified in 48 (55.8%), 36 (41.9%) and 2 (2.3%) isolates, respectively. The obtained RFLP patterns of M. tuberculosis isolates from both the HIV-seropositive and -seronegative groups were variable, suggesting no obvious clustering among the isolates. Similar results were obtained in isolates of M. avium.

Conclusions

This is the first report on the molecular epidemiology of Mycobacterium spp. isolated from HIV-seropositive patients in Japan. The results indicate that no particular clones of M. tuberculosis or M. avium prevail in HIV-seropositive patients in Japan. Further monitoring of mycobacterial infection associated with HIV infection in Japan should be continued.

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Keywords : Mycobacterial infection, HIV-seropositive patients, RFLP

Plan

Introduction

Materials and methods

Bacterial isolates and clinical data

Mycobacterial culture and identification of strains

Drug sensitivity testing

DNA fingerprinting

Analysis

Results

Mycobacterial infection in HIV-seropositive patients

Profile of HIV-seropositive patients with M. tuberculosis

RFLP analysis of M. tuberculosis

Comparison of RFLP patterns between HIV-seropositive and -seronegative TB patients

Profile of HIV-seropositive patients with M. avium infection

RFLP analysis of M. avium

Discussion

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Vol 51 - N° 5

P. 364-374 - décembre 2005 Retour au numéro

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Survey of human immunodeficiency virus (HIV)-seropositive patients with mycobacterial infection in Japan - 14/08/11

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