The genetic portrait of an outbreak strain - 11/08/11

Doi : 10.1016/j.tube.2005.08.019

Hasan Yesilkaya ^a,^⁎ , Ken J. Forbes ^b, Jamila Shafi ^a, Rebecca Smith ^a, Jeremy W. Dale ^c, Kumar Rajakumar ^a,^d, Michael R. Barer ^a,^d, Peter W. Andrew ^a
^a Department of Infection, Immunity and Inflammation, Leicester Medical School, University of Leicester, Leicester LE1 9HN, UK
^b Department of Medical Microbiology, University of Aberdeen, Medical School Building, Foresterhill, Aberdeen AB25 2ZD, UK
^c Microbial Sciences Group, School of Biomedical and Molecular Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK
^d Department of Clinical Microbiology, University Hospitals of Leicester NHS Trust, Leicester, LE1 5WW, UK

Corresponding author. Tel.: +441162231556; fax: +441162525030.

Summary

Unique events in the genome of Mycobacterium tuberculosis, including deletions and IS6110 insertions, have been proposed to be responsible for the virulence phenotype of outbreak strains. Based on this premise, we determined ten IS6110 insertion sites in the genome of the M. tuberculosis CH strain, which was responsible for a large outbreak in Leicestershire, England. Together with previous data on genomic deletions, it was found that 16 genes were mutated either by IS6110 insertions or deletions. The likely impact of these genomic events on the phenotype of the CH strain is discussed.

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Keywords : Mycobacterium tuberculosis, CH strain, Outbreak, Insertion, Deletion, IS6110

Plan

Introduction

Materials and methods

Growth conditions and DNA extraction

IS6110 insertion site detection

Data analysis

Results and discussion

IS6110 mediated genomic events

Functional analysis of major genomic events

Genes mutated by IS6110 insertions and deletions

Conclusions

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Vol 86 - N° 5

P. 357-362 - septembre 2006 Retour au numéro

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The genetic portrait of an outbreak strain - 11/08/11

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