Kinetics of inflammatory cytokines in patients with scrub typhus receiving doxycycline treatment - 08/08/11
, Young Seok Lee, Seung Soon Lee
Corresponding author. Present address: Division of Infectious Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, South Korea. Tel.: +82 2 3410 0323; fax: +82 2 3410 0041.Summary |
Objectives |
Animal models have suggested that various cytokines and chemokines play an important role in host defense against Orientia tsutsugamushi, however, human data are limited. We investigated the kinetics of inflammatory responses in patients with scrub typhus receiving doxycycline.
Methods |
Amongst patients being treated for scrub typhus, serially collected sera were tested for TNF-⍺, IFN-γ, IL-2, IL-4, IL-5, and IL-10 by immunoassay. The mRNAs encoding TNF-⍺, IL-1β, IL-6, IL-8, IFN-γ, IL-12, IL-2, IL-4, IL-5, and IL-10 were measured by semiquantitative reverse transcription-PCR.
Results |
The concentrations of TNF-⍺, IFN-γ, and IL-10 of patients prior to doxycycline treatment were significantly higher than those of healthy volunteers. They decreased markedly within 24h after starting doxycycline. The mRNAs for IL-1β, TNF-⍺, IL-6, IFN-γ, and IL-10 were highly expressed. Expression of mRNAs for IL-1β, IFN-γ, and IL-10 decreased at day 2–7 of doxycycline treatment.
Conclusions |
Inflammatory cytokines including TNF-⍺, IL-1β, and IL-6 are markedly upregulated in patients with scrub typhus. Doxycycline treatment rapidly reduces the production of these cytokines, corresponding to the early defervescence after the start of the treatment. The profiles of T cell-derived cytokines in patients with scrub typhus do not follow typical Th1 or Th2 patterns.
Le texte complet de cet article est disponible en PDF.Keywords : Scrub typhus, Orientia tsutsugamushi, Cytokines, TNF-⍺, Doxycycline
Plan
Vol 56 - N° 1
P. 44-50 - janvier 2008 Retour au numéro
Article précédent
- Antiretroviral therapy in AIDS patients with CMV disease: Impact on the survival and long-term treatment outcome
- Somnuek Sungkanuparph, Taywin Chakriyanuyok, Bodin Butthum
- A comparison of the effectiveness of zanamivir and oseltamivir for the treatment of influenza A and B
- Naoki Kawai, Hideyuki Ikematsu, Norio Iwaki, Tetsunari Maeda, Hideo Kanazawa, Takashi Kawashima, Osame Tanaka, Satoshi Yamauchi, Kenichi Kawamura, Toru Nagai, Satsuki Horii, Nobuo Hirotsu, Seizaburo Kashiwagi
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?