HIV-1 DNA load analysis in peripheral blood lymphocytes and monocytes from naïve and HAART-treated individuals - 08/08/11
, Marco Borderi b, Elisa De Crignis a, Ronny Cicola a, Laura Cimatti a, Francesca Vitone a, Francesco Chiodo b, Maria Carla Re a
Corresponding author. Tel.: +39 051 429 0900; fax: +39 051 307307.Summary |
Objective |
To evaluate HIV-1 DNA load in PBLs and monocytes from both long-term HAART-treated and antiretroviral naïve HIV-1 infected patients.
Methods |
Cross-sectional quantitative analysis of HIV-1 DNA load was performed in PBLs and monocytes, purified from 34 long-term HAART-treated and 34 naïve HIV-1 infected patients, and compared to RNA viral load and CD4+ cell count.
Results |
HAART-treated patients showed significantly lower levels of viral DNA both in PBLs and monocytes in comparison with naïve individuals. Variable levels of HIV-1 DNA amount in monocytes were detected in all naïve patients but only in 12 of 34 HAART-treated individuals. PBLs HIV-1 DNA load was inversely correlated to CD4+ cell count in naïve and HAART-treated patients whereas no association was detected in monocytes.
Conclusions |
Long-term HAART decreased HIV-1 DNA load in PBLs and monocytes demonstrating a valuable inhibitor effect, especially in short-lived reservoirs. In addition, the positive correlation of DNA burden between PBLs and monocytes may suggest a dynamic relation between these reservoirs in the course of disease. HIV-1 DNA load quantitative analysis in PBLs and monocytes may be considered an important approach to study the HIV-1 reservoir and the effectiveness of HAART therapy in HIV-1 seropositive patients.
Le texte complet de cet article est disponible en PDF.Keywords : HIV-1, Peripheral blood lymphocytes, Monocytes, Reservoir, Highly active antiretroviral therapy
Plan
Vol 56 - N° 3
P. 219-225 - mars 2008 Retour au numéro
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