β-Haemolytic group A streptococci emm75 carrying altered pyrogenic exotoxin A linked to scarlet fever in adults - 08/08/11
Summary |
Objective |
To determine the etiological cause of a food-borne outbreak of scarlet fever in adults.
Methods |
Swabs from the throats of the patients and asymptomatic control were cultured on blood agar plates individually. Biochemical identification of all isolates was performed with a VITEX automated system. Antibiotic susceptibility was examined by using the Kirby–Bauer disc diffusion method. emm gene and extracellular pyrogenic exotoxins of each isolate were amplified by using polymerase chain reaction and subjected to DNA sequencing. Sequence differences between the isolated and the highly similar reference sequences were compared on BLAST. Bioinformatics was used to predict protein structures.
Results |
β-Haemolytic group A streptococci (GAS) emm75 were identified from 10 of 13 available patients. The isolates were susceptible to penicillin, ampicillin, vancomycin, cefatriaxone, ofloxacin, linezolid and quinupristin. All of the isolates carried pyrogenic exotoxin A (speA) and cysteine protease (speB). Isolated speA was phylogenetically different from 30 highly similar references on BLAST. Differences in the primary sequence of the deduced protein were 14.37–20.12% between the speA and each of 11 references. Secondary protein structure of the speA was different from the references at the N-terminal.
Conclusions |
GAS emm75 encoding altered speA was responsible for the food-borne outbreak of scarlet fever in adults.
Le texte complet de cet article est disponible en PDF.Keywords : β-Haemolytic group A streptococci, Genotype, Exotoxin, Phylogenesis, Scarlet fever, Food-borne
Plan
Vol 56 - N° 4
P. 261-267 - avril 2008 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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