Immune response to Leishmania antigen in anthroponotic cutaneous leishmaniasis - 07/08/11
Summary |
Background |
Leishmania (L.) tropica is the causative agent of anthroponotic cutaneous leishmaniasis (ACL) in Iran. The disease often heals within a year; however, the non-healing forms of disease are also known. The immunologic responses to L. major infection have been studied in depth, however little is known about the immune status of L. tropica-infected patients.
Materials and methods |
This study was conducted to evaluate T-cell responses to Leishmania antigen in non-healing patients, patients with acute lesion, and healthy donors. Peripheral blood mononuclear cells (PBMC) were cultured with antigen and lymphoproliferative responses were determined. Cytokine profile including gamma interferon (IFN-γ), interleukin (IL)-5, and IL-13 in supernatants of stimulated cells was also determined.
Results |
The results showed PBMC from both groups of patients proliferated vigorously in response to Leishmania antigens. The levels of IFN-γ and IL-13 were comparable between patients with acute lesions and non-healing patients. Non-healing patients had significantly higher median levels of IL-5 than patients with acute lesions. The cells from healthy individuals did not respond to Leishmania antigens.
Conclusions |
High levels of IFN-γ, IL-5, and IL-13 in non-healing patients suggest a mixed Th1/Th2 response, whereas patients with acute lesion respond to infection by Th1-type response.
Le texte complet de cet article est disponible en PDF.Keywords : Immune response, Leishmania tropica, Non-healing patients
Plan
Vol 59 - N° 2
P. 139-143 - août 2009 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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