Characteristics of lymphocyte subsets in HIV-infected, long-term nonprogressor, and healthy Asian children through 12 years of age - 07/08/11
, Tanakorn Apornpong, MSc a, Pope Kosalaraksa, MD d, Tanyathip Jaimulwong, BSc a, Rawiwan Hansudewechakul, MD e, Chitsanu Pancharoen, MD c, Torsak Bunupuradah, MD a, Mom Chandara, MSc f, Thanyawee Puthanakit, MD a, c, Chaiwat Ngampiyasakul, MD g, Jurai Wongsawat, MD h, Suparat Kanjanavanit, MD i, Wicharn Luesomboon, MD j, Phennapha Klangsinsirikul, PhD k, Nicole Ngo-Giang-Huong, PharmD, PhD l, Stephen J. Kerr, PhD a, m, Sasiwimol Ubolyam, MSc a, Tawan Mengthaisong, RN a, Rebecca S. Gelman, PhD n, o, Kovit Pattanapanyasat, PhD p, Vonthanak Saphonn, MD, PhD f, q, Kiat Ruxrungtham, MD, MSc a, c, William T. Shearer, MD, PhD rPREDICT Study Group
Reprint requests: Jintanat Ananworanich, MD, PhD, HIV-NAT, Thai Red Cross AIDS Research Center, 104 Rajdumri Rd, Pathumwan, Bangkok, Thailand 10330.Abstract |
Background |
There are limited data on the immune profiles of HIV-positive children compared with healthy controls, and no such data for Asian children.
Objectives |
To immunophenotype HIV-positive Asian children, including long-term nonprogressors (LTNPs), compared with age-matched healthy controls.
Methods |
We used flow cytometry to analyze 13 lymphocyte and monocyte subsets from 222 untreated, HIV-positive children with 15% to 24% CD4+ T cells and no AIDS-related illnesses and 142 healthy children (controls). Data were compared among age categories. Profiles from LTNPs (n = 50), defined as children ≥8 years old with CD4+ T-cell counts ≥350 cells/mm3, were compared with data from age-matched non-LTNPs (n = 17) and controls (n = 53).
Results |
Compared with controls, HIV-positive children had lower values (cell count per mm3 and percent distribution) for TH cells and higher values for cytotoxic T cells, with reductions in populations of naive TH and cytotoxic T cells, B cells, and natural killer (NK) cells. HIV-positive children had high values for activated TH and cytotoxic T cells. Compared with non-LTNPs, LTNPs had higher values of TH and cytotoxic T cells, naive and memory T-cell subsets, and B and NK cells. Surprisingly, counts of activated TH and cytotoxic T cells were also higher among LTNPs. LNTPs were more frequently male.
Conclusion |
Untreated, HIV-infected Asian children have immune profiles that differ from those of controls, characterized by low values for TH cells, naive T cells, B cells, and NK cells but high values for cytotoxic, activated TH, and cytotoxic T cells. The higher values for activated T cells observed in LTNPs require confirmation in longitudinal studies.
Le texte complet de cet article est disponible en PDF.Key words : HIV, children, lymphocyte, monocyte, phenotyping, long-term nonprogressors, antiretroviral therapy, Asia, disease progression, pediatric AIDS
Abbreviations used : ART, CDC, IQR, LTNP, NK, OR, PREDICT
Plan
| The PREDICT study was sponsored by the National Institute of Allergy and Infectious Diseases, grant no. U19 AI053741, Clinical%20trial.gov identification number NCT00234091. The lymphocyte subset study in HIV-positive children was sponsored by the National Institute of Allergy and Infectious Disease, grant no. 1R01AI075408-0. The Thai Research Council sponsored the study in healthy children. K.P. is a Thailand Research Fund-Senior Research Scholar. Antiretroviral therapy for PREDICT was provided by GlaxoSmithKline, Boehringer Ingelheim, Merck, Abbott, and Roche. |
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| Disclosure of potential conflict of interest: K. Ruxrungtham has received research support from the Division of AIDS, National Institute of Allergy and Infectious Disease, NIH. The rest of the authors have declared that they have no conflict of interest. |
Vol 126 - N° 6
P. 1294 - décembre 2010 Retour au numéro
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