Food allergy herbal formula 2 protection against peanut anaphylactic reaction is via inhibition of mast cells and basophils - 07/08/11
, Chunfeng Qu, PhD a, , Kamal Srivastava, MPhil a, Nan Yang, PhD a, Paula Busse, MD a, Wei Zhao, MD, PhD b, Xiu-Min Li, MD a, ⁎ 
Reprint requests: Xiu-Min Li, MD, Pediatric Allergy and Immunology, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029-6574.Abstract |
Background |
Mast cells and basophils are key effector cells of IgE-mediated anaphylactic reactions. The Chinese herbal formula, food allergy herbal formula 2 (FAHF-2), protects against peanut anaphylaxis in mice. However, the mechanisms underlying this effect are not fully elucidated.
Objective |
To investigate whether FAHF-2 inhibits mast cell/basophil numbers and IgE-mediated activation.
Methods |
Mice with peanut allergy (PNA mice) were treated with FAHF-2 intragastrically for 7 weeks and challenged intragastrically with peanut 1 day and 4 weeks posttreatment. Peripheral blood basophil numbers and peritoneal mast cell numbers and FcεRI expression were determined. Direct effects of FAHF-2 on the murine mast cell line MC/9, and effects of 4 fractions and 3 compounds isolated from FAHF-2 on rat basophilic leukemia cells (RBL-2H3) and human skin mast cells degranulation and on the IgE-mediated spleen tyrosine kinase signaling pathway, were determined.
Results |
Although all sham-treated PNA mice developed anaphylaxis, FAHF-2–treated PNA mice were protected against anaphylaxis after peanut challenge at 1 day and 4 weeks posttherapy. Reduction of peripheral blood basophils began after 1 week of treatment and continued for at least 4 weeks posttherapy. The number and FcεRI expression of peritoneal mast cells were also significantly decreased 4 weeks posttherapy. FAHF-2–treated MC/9 cells showed significantly reduced IgE-induced FcεRI expression, FcεRI γ mRNA subunit expression, proliferation, and histamine release on challenge. Fraction 2 from FAHF-2 inhibited RBL-2H3 cell and human mast cell degranulation. Three compounds from fraction 2—berberine, palmatine, and jatrorrhizine—inhibited RBL-2H3 cell degranulation via suppressing spleen tyrosine kinase phosphorylation.
Conclusion |
Food allergy herbal formula 2 reduction of basophils and mast cell numbers as well as suppression of IgE-mediated mast cell activation may contribute to FAHF-2’s persistent protection against peanut anaphylaxis.
Le texte complet de cet article est disponible en PDF.Key words : Peanut anaphylaxis, Chinese herbal medicine, mast cells/basophils, FcεRI
Abbreviations used : CT, DMEM, FAHF-2, FITC, MFI, PBLC, PE, prep-HPLC, qPCR, RBL-2H3, Syk
Plan
| Supported by the Dugan Family Foundation, the Food Allergy Initiative, and National Institutes of Health grant no. 1R01AT001495-01A1 and no. 2 R01 AT001495-05A1 to X.-M.L. |
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| Disclosure of potential conflict of interest: P. Busse is a consultant for ViroPharma, receives research support from the AAAAI and the NIH, and has provided legal consultation services/expert witness testimony in cases related to hereditary angioedema. W. Zhao receives research support from the NIH. X.-M. Li is a consultant for the Food Allergy Initiative, has received research support from the Food Allergy Initiative and the NIH, and has shares of US patent PCT/US05/08600 on FAHF-2 and Herbal Springs LLC. The rest of the authors have declared that they have no conflict of interest. |
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| A US provisional patent application regarding FAHF-2 (reference no. 60554775) has been filed by X.-M. Li and Herbal Springs LLC. |
Vol 126 - N° 6
P. 1208 - décembre 2010 Retour au numéro
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