IFN-γ, but not IP-10, MCP-2 or IL-2 response to RD1 selected peptides associates to active tuberculosis - 07/08/11
, Alamelu Raja b, Basirudeen Syed Ahamed Kabeer b, Camilla Rodrigues c, Archana Sodha c, Ornella Butera a, Stefania Carrara a, Guy Vernet d, Christophe Longuet d, Giuseppe Ippolito e, Satheesh Thangaraj f, Marc Leportier f, Enrico Girardi g, Philippe Henri Lagrange h
Corresponding author. Tel.: +39 06 55170 906 (954); fax: +39 06 5582 825.Summary |
Objectives |
To evaluate whether in vitro response to Mycobacterium tuberculosis RD1 peptides selected by computational analysis, measured by IFN-γ, IP-10, MCP-2 or IL-2 production, is associated with active tuberculosis (TB) in a country with a high incidence of TB.
Methods |
129 individuals were prospectively enrolled, 41 with active-pulmonary TB and 88 without (household contacts and community controls). A whole blood assay based on RD1 selected peptides was performed. Soluble factors were evaluated by ELISA in plasma harvested at day1-post-culture. Enrolled individuals were also tested by QuantiFERON TB-Gold In tube (QFT-IT) and tuberculin skin tests (TST).
Results |
IFN-γ response to RD1 selected peptides was significantly higher in active TB patients than in household contacts and community controls. IP-10 and MCP-2 response did not differ between active TB patients and household contacts, although it was higher in these groups compared to community controls; conversely IL-2 response did not differ among the three groups. When IFN-γ response to RD1 selected peptides was scored based on receiver-operator-characteristic analysis, active TB was predicted with 68% sensitivity and 86% specificity. QFT-IT and TST showed a sensitivity for active TB of 90% and 68% and a specificity of 58% and 59%, respectively.
Conclusions |
IFN-γ (but not IP-10, MCP-2 and IL-2) response to RD1 selected peptides is associated with active TB with a higher specificity than QFT-IT and TST.
Le texte complet de cet article est disponible en PDF.Keywords : Tuberculosis, LTBI, IGRA, RD1 peptides, IP-10, MCP-2, IL-2
Plan
Vol 61 - N° 2
P. 133-143 - août 2010 Retour au numéro
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