Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots - 06/08/11
Reprint requests: Leland W. K. Chung, Ph.D., Department of Urology, Molecular Urology and Therapeutics Program, Winship Cancer Institute, Emory University School of Medicine, 1365b Clifton Road Northeast, Atlanta, GA 30322Résumé |
Objectives |
To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo.
Methods |
Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System.
Results |
As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors.
Conclusions |
Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.
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| This study was supported by grants from the National Institutes of Health (grant P01 CA098912 to L. W. K. Chung and grants R01 GM60562 and RO1 CA108468 to S. Nie), Department of Defense (grant 17-03-2-0033 to L. W. K. Chung), Georgia Cancer Coalition Distinguished Cancer Scholar Awards (L. W. K. Chung and S. Nie); and in part by grants NKBRP2005CB522605, SKLZZ200810, NFSC30400188, FANEDD200777, IRT0712, and CSTC2008BB5023 from China (C. Shi and Y. Su). |
Vol 74 - N° 2
P. 446-451 - août 2009 Retour au numéro
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