Methodology and pharmacological analysis of effects of uterotonic compounds in human myometrium in vitro - 03/08/11
Reprints: John J. Morrison, MD, FRCOG, Department of Obstetrics and Gynecology, Clinical Science Institute, University College Hospital Galway, Galway, IrelandRésumé |
Objective |
The methodology used to evaluate contractile effects of uterotonic agents in human myometrium in vitro varies. The are no studies evaluating the reliability of these commonly used techniques.
Study Design |
Myometrial strips (n = 72) were exposed to 3 known uterotonic agents: oxytocin, U46619, and phenylephrine. The negative log of the molar concentration of the agonist that produces a half-maximal response (pEC50) and maximal response values were obtained, and compared, when either amplitude or mean force was used as indices of contraction. All data were expressed as a percentage of KCl elicited actvity.
Results |
Using pEC50 measurements, the order of potency was oxytocin greater than U46619 greater than phenylephrine for both indices, whereas the order of maximal response varied between mean force and amplitude. The coefficient of variation was lowest for pEC50 measurements, highest for maximal force estimations, and overall was 10-48% between, and 2-27% within, donor samples.
Conclusion |
These findings support the use of pEC50 measurements for in vitro experiments using uterotonic agents and outline the variability that occurs for such myometrial experiments.
Le texte complet de cet article est disponible en PDF.Key words : human myometrium, maximal response, oxytocin, phenylephrine, U46619, uterotonic agents
Plan
| This study was supported in part by the National University of Ireland Galway and McMaster University. |
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| Cite this article as: Crankshaw DJ, Morrison JJ. Methodology and pharmacological analysis of effects of uterotonic compounds in human myometrium in vitro. Am J Obstet Gynecol 2011;205:155.e1-6. |
Vol 205 - N° 2
P. 155.e1-155.e6 - août 2011 Retour au numéro
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