Simple ultraviolet and high-performance liquid chromatography methods for the evaluation of sunscreen efficacy - 28/07/11
Abstract |
Background |
To prevent DNA damage caused by the ultraviolet (UV) radiation of sunlight, sunscreens are commonly used to protect human skin. Current analysis of sunscreens' effectiveness is done through complicated procedures, including human exposure.
Objective |
We sought to design a simple system using thymidine-thymidine (TT) dinucleotides to analyze the effectiveness of sunscreens.
Methods |
We can directly analyze sunscreen effectiveness and the formation of TT photolesions simply by using UV spectrophotometry and high-performance liquid chromatography (HPLC). Efficient sunscreen has protective effects against UV irradiation damage.
Results |
We have developed a simple method using TT dinucleotide, UV, and HPLC for the analysis of sunscreen effectiveness. Our research indicates that the analytical results from UV are consistent with those of HPLC, which is used to monitor the formation of the TT photolesions. Moreover, both UV and HPLC analyses indicate that TT dinucleotides are better protected against UV damage, using the sunscreens with higher UVB sun protection factor (SPF) value, and that sunscreens with higher SPF lead to reduced photolesion formation. Our UV and HPLC analyses confirm the SPF grading of commercial sunscreens.
Limitations |
In this experiment, only sunscreens were tested. The experiment, therefore, does not apply to other commercial products, such as cosmetic materials that claim UV protection as a secondary benefit.
Conclusion |
In conclusion, we have established a simple strategy to analyze the effectiveness of sunscreens and the quality of these potential cancer-preventive products.
Le texte complet de cet article est disponible en PDF.Key words : high-performance liquid chromatography, method to analyze sunscreen, sunlight, sunscreens, thymidine-thymidine dinucleotide, ultraviolet, ultraviolet irradiation, ultraviolet photolesion
Abbreviations used : HPLC, SPF, UV, TT
Plan
Supported by the Georgia Cancer Coalition Distinguished Cancer Clinicians and Scientists Award (to Dr Huang), a fellowship from the Molecular Basis of Disease Program Area of Focus (Ms Maw), and the Biotechnology Scholars Program of Georgia State University. |
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Conflicts of interest: None declared. |
Vol 65 - N° 2
P. 328-335 - août 2011 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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