Natural killer activity of peripheral-blood mononuclear cells in breast cancer patients - 30/10/09

Doi : 10.1016/j.biopha.2009.02.003

Md. Zahidunnabi Dewan ^a,^b,^{^{1
M.Z. Dewan and M. Takada equally contribute to this report.},}^{^{2
Present address: Department of Pathology, New York University School of Medicine, 550 First Avenue, New York, NY 10016, USA.}}, Masahiro Takada ^c,^{^{1
M.Z. Dewan and M. Takada equally contribute to this report.}}, Hiroshi Terunuma ^d,^e, Xuewen Deng ^d, Sunjida Ahmed ^a, Naoki Yamamoto ^a,^b, Masakazu Toi ^c,^⁎
^a Department of Molecular Virology, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan
^b AIDS Research Center, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
^c Department of Breast Surgery, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
^d Biotherapy Institute of Japan, 2-4-8 Edagawa, Koutou-ku, Tokyo 135-0051, Japan
^e Tokyo Clinic Marunouchi Oazo mc, Shin-Marunouchi Center Building, 1-6-2 Marunouchi, Chiyoda-ku, Tokyo 100-0005, Japan

Corresponding author at: Department of Breast Surgery, Graduate School of Medicine, Kyoto University, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

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Abstract

Natural killer (NK) activity of immune cells plays a central role in host defense against cancer and virus-infected cells. Natural cytotoxic activity of peripheral-blood mononuclear cells was assessed by a Calcein-AM release assay in 89 subjects. In the present study, we here demonstrated that NK activities of peripheral-blood mononuclear cells (PBMCs) from breast cancer patients were significantly lower as compared with that of healthy individuals. There were significant differences in the NK activities of PBMCs from HER2-negative breast cancer patients as compared with HER2-positive patients. Our results suggest that NK activity of PBMCs is lower in breast cancer indicating a role for immunological natural host defense mechanisms against cancer.

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Keywords : NK activity, Breast cancer, NK cell, PBMCs

Plan

Introduction

Materials and methods

Collection of human specimens and isolation of PBMCs

Culture of cell line

Cytotoxic activity

Statistical analysis

Results and discussion

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Vol 63 - N° 9

P. 703-706 - novembre 2009 Retour au numéro

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Natural killer activity of peripheral-blood mononuclear cells in breast cancer patients - 30/10/09

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