Paeoniflorin suppress NF-κB activation through modulation of IκBα and enhances 5-fluorouracil-induced apoptosis in human gastric carcinoma cells - 23/10/08
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Abstract |
Objective |
We sought to determine whether paeoniflorin enhances 5-fluorouracil-induced apoptosis in human gastric carcinoma cells, and, if so, to determine the relationship between this apoptosis and NF-κB activation.
Methods |
Paeoniflorin was diluted to different concentrations and added to gastric carcinoma cells (SGC-7901) at different times. Western blot was used to test the expression of NF-κB in nuclear and IκB
, p-IκB, IKK in cytoplasm. Further, the intranuclear expression of NF-κB was confirmed by ELISA assay. The impact of paeoniflorin and 5-fluorouracil on cell apoptosis of gastric carcinoma cells was estimated by flow cytometry.
Results |
Paeoniflorin revealed dramatic inhibition of NF-κB activity in the nuclei of the cells. The inhibition pattern of NF-κB was exhibited in a time- and dose-dependent manner, which was confirmed by Western blot and ELISA. Decreased nuclear translocation of NF-κB induced by paeoniflorin was found by preventing IκB phosphorylation. Moreover, 5-fluorouracil-induced cell apoptosis was promoted by paeoniflorin in gastric carcinoma cells.
Conclusions |
Paeoniflorin can inhibit NF-κB activity of SGC-7901 cells, and enhance 5-fluorouracil-induced apoptosis of gastric carcinoma cells.
Le texte complet de cet article est disponible en PDF.Keywords : Paeoniflorin, NF-κB, IκB, Apoptosis, Gastric carcinoma cells
Plan
Vol 62 - N° 9
P. 659-666 - novembre 2008 Retour au numéro
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