Study on the repair function of radiation-induced salivary gland injury using human amniotic mesenchymal stem cells pre treated with hypoxia - 15/02/25

Doi : 10.1016/j.jormas.2025.102223

Yingxin Wang ^a,^{^{1
They did equal collaboration to this work.}}, Nana Liu ^b,^{^{1
They did equal collaboration to this work.}}, Xin Zhang ^a, Min Dai ^c, Nini Zhang ^c,^⁎ , Guilin Huang ^d,^⁎
^a Department of Oral Maxillofacial Surgery, Suzhou Stomatological Hospital, Suzhou 215004, PR China
^b Department of Periodontology, Suzhou Stomatological Hospital, Suzhou 215004, PR China
^c Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Zunyi Medical University, Zunyi City, Guizhou Province 563099, PR China
^d Department of Stomatology, the Fifth Affiliated Hospital of Zunyi Medical University, Zhuhai 519090, PR China

^⁎Correspondence author at: Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Zunyi Medical University, No. 143 Dalian Road, Huichuan District, Zunyi City, Guizhou Province 563099, PR China.Department of Oral Maxillofacial Surgery, School and Hospital of Stomatology, Zunyi Medical UniversityNo. 143 Dalian Road, Huichuan DistrictZunyi CityGuizhou Province563099PR China^⁎⁎Correspondence author at: Department of Stomatology, the Fifth Affiliated Hospital of Zunyi Medical University, No. 368 Jinwan Road, Jinhaihai Community, Sanzao Town, Jinwan District, Zhuhai City, Guangdong Province.Department of Stomatology, the Fifth Affiliated Hospital of Zunyi Medical UniversityNo. 368 Jinwan Road, Jinhaihai Community, Sanzao Town, Jinwan DistrictZhuhai CityGuangdong Province

Sous presse. Épreuves corrigées par l'auteur. Disponible en ligne depuis le Saturday 15 February 2025

Abstract

Objective

To investigate the reparative effect of hypoxia pretreated hAMSCs on radiation-induced damage to salivary gland function in mice.

Methods

hAMSCs were separated from human amniotic tissues by mechanical and enzymatic digestion methods and a 15 Gy electron beam was used to locally irradiate the neck of mouse to create a salivary gland injury model. The mouse models were randomly divided into four groups: control group, IR+PBS group, IR+Nor group and IR+HP group.

Result

Two months after hAMSCs injection, the saliva flow of mice in the IR+PBS group was significantly lower than that of the control group (P < 0.05). The saliva flow of mice in the IR+Nor group and IR+HP group were significantly increased compared to the IR+PBS group (P < 0.05). The cell apoptosis rate of the IR+PBS group was sensibly higher than that of the blank control group (P < 0.05). The cell apoptosis rates of the IR+Nor group and the IR+HP group were lower than that of the IR+PBS group. In addition, the apoptosis rate of the IR+HP group was lower than that of the IR+Nor group (P < 0.05). The changes of IOD of α-Amy in each group showed that the expression of α - Amy in the IR+PBS group was significantly lower than that in the blank control group (P < 0.05). Compared with the IR+PBS group, the IR+Nor group and the IR+HP group showed an obvious increase in the expression of α-Amy (P < 0.05).