A client presented a 3-year-old dog to the clinic with the primary complaint of recurrent scrotal enlargement and a request for castration. The dog had a history of infertility, recurrent scrotal enlargement and otitis externa. Microbial culture of scrotal subcutaneous fluid revealed heavy growth of Staphylococcus aureus. The scrotal tissue was histopathologically confirmed to be a scrotal granuloma associated with hypospermatogenesis and focal areas of epididymal tubular hyperplasia. The final diagnosis made was scrotal granuloma from chronic S. aureus infection. There is scarce literature on scrotal granuloma associated with S. aureus infection and infertility in dogs as against several cases in humans. There is often an indiscriminate preference for removing the testes and scrotum of dogs presenting chronic disease conditions of the testes and scrotum, unlike in humans. The case was managed by the removal of the testes and scrotum (orchiectomy and scrotal ablation). The dog's fertility could have been salvaged by testicular sperm extraction and in-vitro fertilisation but, the technology was not available in the dog's current location. This case reveals a need for Theriogenologists in developing countries to develop or adopt skills in testicular sperm harvest and in-vitro fertilisation of spermatozoa from hypo-spermatogenic patients.