Clonal outbreak of Candida vulturna in a paediatric oncology ward in Maranhão, Brazil - 06/12/24
Summary |
Objective |
To describe an outbreak due to Candida vulturna, a newly emerging Candida species belonging to the Candida haemulonii species complex in the Metschnikowiaceae family.
Methods |
In this retrospective cohort study we genotyped 14 C. vulturna bloodstream isolates, occurring in a 4-month-period in paediatric cancer patients in a Brazilian hospital. To prove an outbreak, ITS sequence analysis and whole genome sequencing (WGS) was done. Antifungal susceptibility was performed with the reference CLSI method and the commercial Sensititre YeastOne (SYO) YO10 plates. A control C. vulturna isolate from another region in Brazil was included in all analyses.
Results |
MALDI-TOF-MS identified isolates as C. pseudohaemulonii and C. duobushaemulonii albeit with low scores and therefore molecular methods were required for accurate identification. ITS sequence analyses clearly differentiated C. vulturna from other species in the C. haemulonii species complex. WGS proved the presence of a clonal outbreak with C. vulturna involving 14 paediatric patients. Antifungal susceptibility testing (AFST) with two methods showed the isolates had low MICs of commonly available antifungals.
Conclusion |
This study describes an outbreak due to the rare yeast C. vulturna, related to C. auris, during a four-month period in patients admitted to a paediatric oncology ward in a Brazilian hospital. In contrast to previous studies the yeast was susceptible to all antifungals and patient outcome was good.
Le texte complet de cet article est disponible en PDF.Highlights |
• | This study aimed to diagnose and evaluate an outbreak of Candida vulturna fungaemia. |
• | MALDI-TOF was not able to correctly identify C. vulturna, a close relative of Candida auris. |
• | The isolates involved in this outbreak of 14 patients had low amphotericin B and azoles MICs. |
• | Internal transcribed spacer (ITS) region sequencing was necessary to identify C. vulturna. |
• | Whole genome sequencing of a subset proved a clonal outbreak of C. vulturna. |
Keywords : Fungal outbreak, Genotyping, Rare yeast, Candida vulturna, Candida haemulonii species complex, Whole genome sequencing, Antifungal susceptibility
Plan
Vol 89 - N° 6
Article 106349- décembre 2024 Retour au numéro
Article précédent
- Real-world effectiveness and safety of simnotrelvir/ritonavir for COVID-19: A nationwide, multicenter, prospective, observational cohort study in China
- Bing Han, Chunling Du, Min Deng, Renhong Tang, Jianping Dong, Xu Song, Yunfeng Qiao, Zheng Ni, WenJie Yang, Jiankun Yang, Tianxin Xiang, Yan Huang, Yu Zhong, Zhongfa Zhang, Lisheng Yang, Jikang Yang, Jinghua Wang, Lanbing Zheng, Libing Ma, Zhinan Shou, Ran Cao, Jinghua Ma, Gang He, Jing Yuan, Chongjie Pang, Jing Xu, Jing Huang, Xiaomei Yuan, Yunfeng Wu, Yong Xiong, Xiangjie Zhang, Hongying Liu, Binfeng Gao, Huan Chen, Tengfei Ma, Shuangsuo Dang, Qingyu Zhang, Rui Yuan, Yunqing Wei, Tongbai Xu, Zhulian Deng, Yan Gong, Jianfen Gao, Rongmeng Jiang
- The latform Trial n VID-19 priming and sting (PICOBOO): The immunogenicity, reactogenicity, and safety of licensed COVID-19 vaccinations administered as a second booster in BNT162b2 primed individuals aged 18-<50 and 50-<70 years old
- C. McLeod, M. Dymock, KL Flanagan, M. Plebanski, HS Marshall, MJ Estcourt, U. Wadia, MC Tjiam, CC Blyth, K. Subbarao, FL Mordant, S. Nicholson, N. Cain, R. Brizuela, SN Faust, RB Thornton, Z. Ellis, A. Mckenzie, JA Marsh, TL Snelling, PC Richmond
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?