HLA Class I and Class II Alleles and Haplotypes of Algerian population from Algiers and neighbouring area - 24/11/24

Allèles et haplotypes HLA de classe I et de classe II dans la population algérienne d’Alger et des environs

Doi : 10.1016/j.tracli.2024.11.007

Malika Bouali-Benhalima ^a,^⁎ , Meriem Abbadi ^a, Sarah Hamada ^b, Celine Mandier ^b, Souhila Amoura ^a, Valérie Dubois ^b, Sofiane Samir Salah ^a
^a Immunology department, Mustapha Bacha University Hospital, Algiers, Algeria
^b HLA Laboratory Etablissement Français du Sang Auvergne Rhone Alpes, Lyon, France

^⁎Corresponding author at: Immunology department, Mustapha Bacha University Hospital, Algiers, Algeria.Immunology departmentMustapha Bacha University HospitalAlgiersAlgeria

Sous presse. Manuscrit accepté. Disponible en ligne depuis le Sunday 24 November 2024

Highlights

•	This work is bringing new knowledge about allele frequency and haplotype segregation in the Algerian population. The comparison with other described populations can bring an idea about population migration and closest of the studied population to another.
•	This work had been performed in the Histocompatibility laboratory of CHU Mustapha in Algiers. It was managed by Pr Benhalima.

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Abstract

In this study, we aimed to investigate the current genetic diversity and provide additional insights into the origins of the Algerian population by analyzing the frequencies of HLA -A,-B,-DRB1,-DQB1 alleles and associated haplotypes.

We analyzed 1,082 unrelated healthy Algerian individuals, who were potential kidney donors, recruited and assessed in the Immunology Department of CHU Mustapha in Algiers over a 10-year period (2009 to 2019). HLA genotyping was performed by Polymerase Chain Reaction Sequence Specific Primers (PCR-SSP). The allelic typing estimate and haplotype frequencies were calculated using the R studio® software and Haplotype frequency estimation from the website https://hla-net.eu/.

We identified a total of 20 HLA-A alleles, 31 HLA-B alleles, 13 HLA-DRB1 alleles and5 HLA-DQB1 alleles.

Regarding the allele frequency, the haplotype frequency and the distribution with other population database, we were able to provide a description of Algerian genetic background and draw some anthropogenetic conclusions.

This study of HLA polymorphism in the Algerian population shows a strong genetic similarity with the populations of southern Europe and North Africa (Morocco, Tunisia and Libya) with which it has shared a common history since antiquity.

In addition to its contribution to anthropogenetics, our study, serve as a reference database for studies exploring associations between the HLA system and certain pathologies.

Le texte complet de cet article est disponible en PDF.

Keywords : Algeria, alleles, haplotypes, HLA